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020			_a9781592599073
024	7		_a10.1385/1592599079 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR389 _b2005 EB
245	0	0	_aHuman Retrovirus Protocols : _bVirology and Molecular Biology _cedited by Tuofu Zhu
250			_a1st edition 2005
264		1	_aTotowa, NJ _bHumana Press _c2005
300			_a1 recurso en línea (XIV, 494 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v304
505	0		_aMethods for Virus Isolation and Detection -- Enhanced Culture Assay for Detection and Quantitation of Latently Infected, Resting CD4+ T-Cells Carrying Replication-Competent Virus in HIV-1-Infected Individuals -- Isolation, Propagation, and Titration of Human Immunodeficiency Virus Type 1 From Peripheral Blood of Infected Individuals -- Isolation of Human Immunodeficiency Virus Type 1 From Peripheral Blood Monocytes -- Isolation, Propagation, and HIV-1 Infection of Monocyte-Derived Macrophages and Recovery of Virus From Brain and Cerebrospinal Fluid -- Isolation and HIV-1 Infection of Primary Human Microglia From Fetal and Adult Tissue -- Isolation of Human Immunodeficiency Virus Type 1 From Semen and Vaginal Fluids -- Isolation and Quantification of HIV From Lymph Nodes -- Isolation of Human Immunodeficiency Virus Type 2 Biological Clones From Peripheral Blood Lymphocytes -- Isolation and Confirmation of Human T-Cell Leukemia Virus Type 2 From Peripheral Blood Mononuclear Cells -- Isolation of Foamy Viruses From Peripheral Blood Lymphocytes -- Alu-LTR Real-Time Nested PCR Assay for Quantifying Integrated HIV-1 DNA -- Quantification of HFV-Integrated DNA in Human Cells by Alu-LTR Real-Time PCR -- Detection of HIV-1 Provirus and RNA by In Situ Amplification -- Detection of HTLV-1 Gene on Cytologic Smear Slides -- Detection of HIV-2 by PCR -- Quantitation of HIV-1 Viral RNA in Blood Plasma and Genital Secretions -- Quantification of Proviral DNA Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real-Time PCR -- Plasma RNA Viral Load in HIV-1 Group O Infection by Real-Time PCR -- A New Combined HIV p24 Antigen and Anti-HIV-1/2/O Screening Assay -- A New Automated Fourth-Generation HIV Screening Assay With Sensitive Antigen Detection Module and High Specificity -- Detection of Antiviral Antibodies Using Enzyme-Linked Immunosorbent Assay -- Plaque-Reduction Assays for Human and Simian Immunodeficiency Virus Neutralization -- Detection of Drug-Resistant HIV-1 Strains -- Molecular Biology Methods -- Determination of Cell Tropism of HIV-1 -- Determination of Co-Receptor Usage of HIV-1 -- Quantitative Evaluation of HIV and SIV Co-Receptor Use With GHOST(3) Cell Assay -- Phenotypic Characterization of Blood Monocytes From HIV-Infected Individuals -- Methods to Determine HIV-1 Ex Vivo Fitness -- A Yeast Recombination-Based Cloning System to Produce Chimeric HIV-1 Viruses and Express HIV-1 Genes -- PCR Amplification, Cloning, and Construction of HIV-1 Infectious Molecular Clones From Virtually Full-Length HIV-1 Genomes -- Amplification and Cloning of Near Full-Length HIV-2 Genomes -- Growth and Manipulation of a Human T-Cell Leukemia Virus Type 2 Full-Length Molecular Clone -- Construction and Analysis of Genomic, Full-Length Infectious Foamy Virus DNA Clones -- Molecular Characterization of Proteolytic Processing of the Gag Proteins of Human Spumaretrovirus -- Assessing the Relative Efficacy of Antiretroviral Activity of Different Drugs on Macrophages -- Gene Expression Profiling of HIV-1 Infection Using cDNA Microarrays -- Determination of DC-SIGN and DC-SIGNR Repeat Region Variations.
520			_aAdvances in our understanding of human retroviruses have been occurring at a very rapid pace and have contributed significantly to the development of new biotechnologies. In Human Retrovirus Protocols: Virology and Molecular Biology, recognized international researchers describe in detail the basic and state-of-the-art methods they have optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from proven methods for the isolation and detection of human retroviruses to advanced methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from peripheral blood mononuclear cells (PBMC), blood monocytes, brain tissues, cerebrospinal fluids, semen, the vagina, and lymph nodes. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinant virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection. The protocols follow the highly praised Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Human Retrovirus Protocols: Virology and Molecular Biology offers novice and experienced researchers alike proven tools essential for productive study and understanding of the human retroviruses.
988			_aSpringer_Protocols_2005
650		7	_2embne _9676135 _aVirología molecular _vManuales de laboratorio
650		7	_2embne _9139103 _aBiología molecular _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617375996
776	0	8	_iPrinted edition: _z9781588294951
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592599079 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b01/2024 _dz _eb _zSI