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020 _a9781493925902
024 7 _a10.1007/978-1-4939-2590-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH588 .S83
_b2015 EB
245 0 0 _aStem Cell Renewal and Cell-Cell Communication :
_bMethods and Protocols
_cedited by Kursad Turksen
250 _a1st edition 2015
264 1 _aNew York, NY
_bSpringer International Publishing
_c2015
300 _a1 recurso en línea (X, 210 páginas)
_b44 ilustraciones, 24 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1212
505 0 _aLaboratory Control and Basic Culture Protocols for Stem Cell Self-Renewal -- Advanced Fixation for Transmission Electron Microscopy Unveils Special Extracellular Matrix Within the Renal Stem/Progenitor Cell Niche -- Multiplex Immunoassays for Quantification of Cytokines, Growth Factors and Other Proteins in Stem Cell Communication -- Porous Membrane Culture Method for Expansion of Human Pluripotent Stem Cells -- Tuning Differentiation Signals for Efficient Propagation and In Vitro Validation of Rat Embryonic Stem Cell Cultures -- Generation, Expansion and Differentiation of Human Pluripotent Stem Cell (hPSC) Derived Neural Progenitor Cells (NPCs) -- Isolation, Long-Term Expansion, and Differentiation of Murine Neural Stem Cells -- Generation, Expansion, and Differentiation of Cardiovascular Progenitor Cells from Human Pluripotent Stem Cells -- A Practical Guide for the Isolation and Maintenance of Stem Cells from Tendon -- A Human Colonic Crypt Culture System to Study Regulation of Stem Cell-Driven Tissue Renewal and Physiological Function -- Ca2+ Handling in Mouse Embryonic Stem Cell Derived Cardiomyocytes -- Potential Application of Extracellular Vesicles of Human Adipose Tissue-Derived Mesenchymal Stem Cells in Alzheimer's Disease Therapeutics -- Application of Fluid Mechanical Force to Embryonic Sources of Hemogenic Endothelium and Hematopoietic Stem Cells -- Electrophysiological Recordings from Neuroepithelial Stem Cells -- In Vivo Stem Cell Transplantation Using Reduced Cell Numbers.
520 _aThis volume examines cell-cell interactions and stem cell renewal, two topics that are now inexorably linked as science strives to understand the stem cell niche and its function. Gathering a number of representative protocols, this detailed collection promises to provide readers with approaches for studying these complimentary aspects of stem cells. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and reliable, Stem Cell Renewal and Cell-Cell Communication: Methods and Protocols will aid researchers in using these methods to advance their own studies.
988 _aSpringer_Protocols_2015
650 7 _2embne
_9160528
_aCélulas madre
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781493925896
776 0 8 _iPrinted edition:
_z9781493925919
776 0 8 _iPrinted edition:
_z9781493949397
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2590-2
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b12/2023
_dz
_eb
_zSI