000			04909nam a2200385 i 4500
999			_c393570 _d393570 _x1
001			393570
003			ES-MaUEC
005			20240314174412.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			100301s2006 xxua o |||| 0|eng d
020			_a9781597450485
024	7		_a10.1385/1597450480 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP517 .C45 _b2006 EB
245	0	0	_aTransmembrane Signaling Protocols _cedited by Hydar Ali, Haribabu Bodduluri
250			_a2nd edition 2006
264		1	_aTotowa, NJ _bHumana Press _c2006
300			_a1 recurso en línea (XII, 364 páginas) _b54 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v332
505	0		_aOverviews -- Transmembrane Signaling by G Protein-Coupled Receptors -- Crosstalk Coregulation Mechanisms of G Protein- Coupled Receptors and Receptor Tyrosine Kinases -- Protein-Protein Interactions in Signaling Cascades -- Specific Topics -- Biological Role of the CXCR4-SDF-1 Axis in Normal Human Hematopoietic Cells -- Functional Expression of CXCR4 in Saccharomyces cerevisiae in the Development of Powerful Tools for the Pharmacological Characterization of CXCR4 -- Characterization of Constitutively Active Mutants of G Protein-Coupled Receptors -- G Protein-Coupled Receptor Dimerization and Signaling -- Real-Time Analysis of G Protein-Coupled Receptor Signaling in Live Cells -- Isolation of Membrane Rafts and Signaling Complexes -- Methods for the Study of Signaling Molecules in Membrane Lipid Rafts and Caveolae -- Bioluminescence Resonance Energy Transfer to Monitor Protein-Protein Interactions -- Identification of Interacting Proteins Using the Yeast Two-Hybrid Screen -- Analysis of PDZ Domain Interactions Using Yeast Two-Hybrid and Coimmunoprecipitation Assays -- Clustering Assay for Studying the Interaction of Membrane Proteins with PDZ Domain Proteins -- Mammalian Cell Microinjection Assay to Study the Function of Rho Family Guanosine Triphosphatases -- Affinity-Based Assay of Rho Guanosine Triphosphatase Activation -- Assay of Phospholipase D Activity in Cell-Free Systems -- Reconstitution System Based on Cytosol-Depleted Cells to Study the Regulation of Phospholipase D -- Analysis of Global Gene Expression Profiles Activated by Chemoattractant Receptors -- Genetic Reconstitution of Bone Marrow for the Study of Signal Transduction Ex Vivo -- Proteomic Analysis of Human Neutrophils.
520			_aThe complete sequencing of the human genome and many new microarray and proteomic methods for analyzing global changes in both gene expression and protein profiles have greatly increased our ability to understand transmembrane signaling processes in close detail. In this fully updated and expanded second edition of Transmembrane Signaling Protocols, leading experts demonstrate the use of such techniques as single-cell imaging, bioluminescence resonance energy transfer, and global proteomics in the study of transmembrane signaling events. Highlights include the functional expression and genetic selection of mutant mammalian transmembrane receptors in yeast, real-time analysis of transmembrane receptor signaling in live cells, and bioluminescence energy transfer to monitor protein-protein interaction. Additional chapters use state-of-the-art methods to analyze gene expression and proteomic profiles related to transmembrane signaling events, as well as to genetically reconstitute bone marrow for the study of signal transduction ex vivo. Introductory chapters provide a wide-ranging perspective, including an overview of the many recent advances in our understanding of signal transduction pathways. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Transmembrane Signaling Protocols, Second Edition, makes available to novice and experienced researchers alike the readily reproducible techniques needed to advance the study of signal transduction pathways today.
988			_aSpringer_Protocols_2006
650		7	_2embne _9689361 _aTransducción de la señal celular
776	0	8	_iPrinted edition: _z9781617376337
776	0	8	_iPrinted edition: _z9781588295460
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1597450480 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI