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008			100301s2004 xxu| o |||| 0|eng d
020			_a9781592598168
024	7		_a10.1385/1592598161 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP517 .C45 _b2004 EB
245	0	0	_aSignal Transduction Protocols _cedited by Robert C. Dickson, Michael D. Mendenhall
250			_a2nd edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XII, 327 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v284
505	0		_aMaking Protein Immunoprecipitates -- Signal Transduction Inhibitors in Cellular Function -- Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets -- A High-Throughput Mammalian Cell-Based Transient Transfection Assay -- Determining Protein Half-Lives -- Assaying Protein Kinase Activity -- Comparative Phosphorylation Site Mapping From Gel-Derived Proteins Using a Multidimensional ES/MS-Based Approach -- Studies of Calmodulin-Dependent Regulation -- Measurement of Protein-DNA Interactions In Vivo by Chromatin Immunoprecipitation -- Characterization of Protein-DNA Association In Vivo by Chromatin Immunoprecipitation -- Nonradioactive Methods for Detecting Activation of Ras-Related Small G Proteins -- Nucleocytoplasmic Glycosylation, O-GlcNAc -- Techniques in Protein Methylation -- Assaying Lipid Phosphate Phosphatase Activities -- Assaying Phosphoinositide Phosphatases -- Assaying Phospholipase A2 Activity -- Measurement and Immunofluorescence of Cellular Phosphoinositides -- Measuring Dynamic Changes in cAMP Using Fluorescence Resonance Energy Transfer -- In Vivo Detection of Protein-Protein Interaction in Plant Cells Using BRET -- Revealing Protein Dynamics by Photobleaching Techniques -- Assaying Cytochrome c Translocation During Apoptosis.
520			_aCarrying on the high standards of the much-acclaimed first edition, highly experienced investigators have extensively updated Signal Transduction Protocols with many of the new approaches that have been transforming the field. Included in this new edition are readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. Wherever possible, protocols for the assay of general classes of signal transduction components have been identified so they can be adapted to the assay of any member of that class. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and eminently practical, Signal Transduction Protocols, 2nd ed. offers novice and experienced researchers alike an indispensable set of standardized, but readily adaptable, approaches for the assay of key signaling molecules-an invaluable tool for all laboratories newly entering the field, expanding the breadth of previous investigations, or seeking to improve an existing approach.
988			_aSpringer_Protocols_2004
650		7	_2embne _9689361 _aTransducción de la señal celular
776	0	8	_iPrinted edition: _z9781617374487
776	0	8	_iPrinted edition: _z9781588292452
776	0	8	_iPrinted edition: _z9781489939708
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592598161 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI