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008			170814s2017 xxua o |||| 0|eng d
020			_a9781493972371
024	7		_a10.1007/978-1-4939-7237-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR185.2 _b2017 EB
245	0	0	_aInnate Antiviral Immunity : _bMethods and Protocols _cedited by Karen Mossman
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 275 páginas) _b41 ilustraciones, 30 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1656
505	0		_aThe Application of Humanized Mouse Models for the Study of Human Exclusive Viruses -- Zebrafish as a Model for the Study of Host-Virus Interactions -- Northern Blot Detection of Virus-Derived Small Interfering RNAs in Caenorhabditis elegans Using Non-Radioactive Oligo Probes -- Extraction and qPCR-Based Detection of miRNAs from Cultured PBMCs of Bubaline Origin -- Visualizing Virus-Derived dsRNA Using Antibody-Independent and -Dependent Methods -- RNA PAMPs as Molecular Tools for Evaluating RIG-I Function in Innate Immunity -- Methods to Visualize MAVS Subcellular Localization -- Purification of Cyclic GMP-AMP from Viruses and Measurement of Its Activity in Cell Culture -- cGAMP Quantification in Virus-Infected Human Monocyte-Derived Cells by HPLC-Coupled Tandem Mass Spectrometry -- Methods of Assessing STING Activation and Trafficking -- Genome-Wide CRISPR/Cas9 Screening for High-Throughput Functional Genomics in Human Cells -- High Throughput Screening for Identification of Novel Innate Immune Activators -- Chromosome Conformation Capture for Research on Innate Antiviral Immunity -- Discovery of Variants Underlying Host Susceptibility to Virus Infection Using Whole-Exome Sequencing -- Isolation, Purification, and Culture of Primary Murine Sensory Neurons -- Isolation of Group 2 Innate Lymphoid Cells from Mouse Lungs -- Epidemiological Methods.
520			_aThis book explores methods to study the complex and evolving interplay between a virus and its host that range from model systems to the detection of chemical molecules. The collection starts with the application of humanized mice and zebrafish as model organisms to study virus-host interactions and induction of innate immune responses. Subsequent chapters outline diverse methods to detect small interfering RNAs, microRNAs, and virus-derived dsRNA from a variety of cells, tissues, and organisms, as well as to interrogating the cytosolic RNA and DNA sensing pathways, including using RNA PAMPs as molecular tools, purification of cGAMP from virus particles and infected cells, and mechanisms to visualize the subcellular localization and activation of the adaptor proteins MAVS and STING. Cutting-edge methods, including high-throughput and genome-wide CRISPR/Cas9 screens, chromosome conformation capture, and whole-exome sequencing, are described to identify novel mediators, pathways, and variants underlying host susceptibility. Given the importance of studying these pathways and players under physiologic conditions, methods describing the isolation of primary mouse sensory neurons and group 2 innate lymphoid cells are also provided. Finally, this collection comes full circle back to the whole organism level and concludes with epidemiological methods to investigate virus-host interactions and the induction of innate immunity. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Innate Antiviral Immunity: Methods and Protocols spans a diverse array of approaches to study and elucidate the intricacies of this vital area of study.
988			_aSpringer_Protocols_2017
650		7	_2embne _9145491 _aInmunidad _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493972364
776	0	8	_iPrinted edition: _z9781493972388
776	0	8	_iPrinted edition: _z9781493984268
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7237-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI