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008			130729s2013 xxua o |||| 0|eng d
020			_a9781627035293
024	7		_a10.1007/978-1-62703-529-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRA1199 _b2013 EB
245	0	0	_aGenotoxicity Assessment : _bMethods and Protocols _cedited by Alok Dhawan, Mahima Bajpayee
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XV, 463 páginas) _b106 ilustraciones, 62 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1044
505	0		_aBacterial Mutation Assays -- In Vitro Mouse Lymphoma (L5178Y Tk+/- -3.7.2.C) Forward Mutation Assay -- Erythrocyte--Based Pig--a Gene Mutation Assay -- Detection of in vivo Mutation in the Hprt and Pig--a Genes of Rat Lymphocytes -- In vivo cII, gpt, and Spi- gene Mutation Assays in Transgenic Mice and Rats -- In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests -- Analysis of Chromosome Aberrations in Somatic and Germ Cells of the Mouse -- Chromosomal Aberration Test in Human Lymphocytes -- In vivo Micronucleus Assay in Mouse Bone Marrow and Peripheral Blood -- Micronucleus Assay in Human Cells: Lymphocytes and Buccal Cells -- Flow Cytometric Determination of Micronucleus Frequency -- Fluorescent in situ Hybridization (FISH) Technique for the Micronucleus Test -- Comparative Genomic Hybridization (CGH) in Genotoxicology -- The in vitro Micronucleus Assay and Kinetochore Staining: Methodology and Criteria for the Accurate Assessment of Genotoxicity and Cytotoxicity -- g--H2AX Detection in Somatic and Germ Cells of Mice -- Multicolour Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers -- The Comet Assay: Assessment of in vitro and in vivo DNA Damage -- The Comet Assay in Human Biomonitoring -- The Comet assay in Marine Animals -- Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo -- 32P--Postlabeling Analysis of DNA Adducts -- Micronucleus Assay with Tetrad Cells of Tradescantia -- The Wing--Spot and the Comet Tests as useful Assays Detecting Genotoxicity in Drosophila -- Genotoxicity Guidelines Recommended by International Conference of Harmonization (ICH).
520			_aGenetic toxicology is recognized by geneticists and researchers concerned with the genetic impact of man-made chemicals. In Genotoxicity Assessment: Methods and Protocols, expert researchers in the field provide comprehensive genetic toxicology protocols. These include in vitro and in vivo protocols on mutation assays, cytogenetic techniques, and primary DNA damage, assays in alternate to animal models, and updated ICH guidelines. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Genotoxicity Assessment: Methods and Protocols seeks to aid research students and scientists working in regulatory toxicology as well as biomedical, biochemical and pharmaceutical sciences.
988			_aSpringer_Protocols_2013
650		7	_2embne _9140328 _aToxicología _vManuales de laboratorio
650		7	_2embne _9140562 _aGenética humana _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781627035286
776	0	8	_iPrinted edition: _z9781627035309
776	0	8	_iPrinted edition: _z9781493963027
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-529-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI