000			04009nam a2200397 i 4500
999			_c393530 _d393530
001			393530
003			ES-MaUEC
005			20231226122851.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			141126s2015 xxua o |||| 0|eng d
020			_a9781493921935
024	7		_a10.1007/978-1-4939-2193-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH324.9 .C7 _b2015 EB
245	0	0	_aCryopreservation and Freeze-Drying Protocols _cedited by Willem F. Wolkers, Harriëtte Oldenhof
250			_a3rd edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XIII, 509 páginas) _b90 ilustraciones, 35 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1257
505	0		_aPrinciples of Cryopreservation -- Principles of Cryopreservation By Vitrification -- Modeling and Optimization of Cryopreservation -- The Principles of Freeze-Drying -- Use of In Situ Fourier Transform Infrared Spectroscopy to Study Freezingand Drying of Cells -- Calorimetric Analysis Of Cryopreservation and Freeze-Drying Formulations -- Measurement Of Intracellular Ice Formation Kinetics By High-Speed Video Cryomicroscopy -- Laser Scanning Microscopy in Cryobiology -- Low Temperature Electron Microscopy -- Cryopreservation of Semen From Domestic Livestock -- Cryopreservation of Mammalian Oocytes -- Vitrification: A Simple And Successful Method for Cryostorage of Human Blastocysts -- Efficient Cryopreservation of Human Pluripotent Stem Cells By Surface-Based Vitrification -- Cryopreservation Of Greenshell™ Mussel (Perna Canaliculus) Sperm -- Membrane Modification Strategies for Cryopreservation -- Sperm Clean-Up and Density Centrifugation Procedures -- Cryopreservation of Red Blood Cells -- Cord Blood Clinical Processing, Cryopreservation,  and Storage -- Directional Freezing For Large Volume Cryopreservation -- Vitrification Of Heart Valve Tissues -- Cryopreservation of Plant Cell Lines -- Writing Standard Operating Procedures (Sops) For Cryostorage Protocols: Using Shoot Meristem Cryopreservation As An Example -- Freeze-Drying Of Proteins -- Freeze-Drying Of Lactic Acid Bacteria -- Freeze-Drying Of Mammalian Sperm -- Freeze-Drying Of Decellularized Heart Valve Tissues.
520			_aThis volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens.
988			_aSpringer_Protocols_2015
650		7	_2embne _9673643 _aCrioconservación de órganos, tejidos, etc.
776	0	8	_iPrinted edition: _z9781493921928
776	0	8	_iPrinted edition: _z9781493921942
776	0	8	_iPrinted edition: _z9781493949120
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2193-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI