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008			130830s2013 xxua o |||| 0|eng d
020			_a9781627034876
024	7		_a10.1007/978-1-62703-487-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552 .M44 _b2013 EB
245	0	0	_aMembrane Biogenesis : _bMethods and Protocols _cedited by Doron Rapaport, Johannes M. Herrmann
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XIV, 404 páginas) _b80 ilustraciones, 48 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1033
505	0		_aQuantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry -- Thin-Layer Chromatography of Phospholipids -- Analysis of Membrane Lipid Biogenesis Pathways Using Yeast Genetics -- Using 2D Crystals to Analyze the Structure of Membrane Proteins -- Crystallization of Membrane Proteins -- Molecular Dynamics Simulations of Membrane Proteins -- Site-Specific Fluorescent Probe Labeling of Mitochondrial Membrane Proteins -- Topology Determination of Untagged Membrane Proteins -- self-assembling GFP: A Versatile Tool for Plant (Membrane) Protein Analyses -- The Use of Cardiolipin-Containing Liposomes as a Model System to Study the Interaction Between Proteins and the Inner Mitochondrial Membrane -- Analysis of the Interaction Between Membrane Proteins and Soluble Binding Partners by Surface Plasmon Resonance -- Peptide Interaction with and Insertion into Membranes -- Scanning Fluorescence Correlation Spectroscopy in Model Membrane Systems -- Analyses of Protein-Protein Interactions by In Vivo Photocrosslinking in Budding Yeast -- Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for the Characterization of Membrane Proteins -- Membrane Partitioning and Translocation Studied by Isothermal Titration Calorimetry -- Analyzing Membrane Dynamics with Live Cell Fluorescence Microscopy with a Focus on Yeast Mitochondria -- Analysis of Protein Translocation into the Endoplasmic Reticulum of Human Cells -- An Assay to Monitor the Membrane Integration of Single-Span Proteins -- Methods to Study the Biogenesis of Membrane Proteins in Yeast Mitochondria -- Reconstitution of Mitochondrial Presequence Translocase into Proteoliposomes -- Single Channel Analysis of Membrane Proteins in Artificial Bilayer Membranes -- Quantification of Protein Complexes by Blue Native Electrophoresis -- Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) and GFP-Fusions.
520			_aMembrane proteins and membrane lipids form complex interactive systems that are highly dynamic and able to be studied only by combinations of different in vivo and in vitro techniques.  In Membrane Biogenesis: Methods and Protocols, experts in the field present a broad collection of methods to study the biogenesis and function of cellular membranes. Beginning with how membrane lipids or membrane proteins can be studied, this detailed volume continues with sections covering different procedures to investigate the interaction of membrane proteins among each other or with membrane lipids, methods to study the biogenesis of membrane proteins and the dynamics of organelles, as well as protocols for the analyses of the functions or complex organization of membrane proteins.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Extensive and easily applicable, Membrane Biogenesis: Methods and Protocols provides readers with a comprehensive but still concise collection including both basic protocols of rather general application and more specialized methods for specific and novel techniques.
988			_aSpringer_Protocols_2013
650		7	_2embne _9671893 _aProteínas de membranas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781627034883
776	0	8	_iPrinted edition: _z9781627034869
776	0	8	_iPrinted edition: _z9781493963041
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-487-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI