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020 _a9781592598601
024 7 _a10.1385/1592598609
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH631
_b2005 EB
245 0 0 _aCell Migration :
_bDevelopmental Methods and Protocols
_cedited by Jun-Lin Guan
250 _a1st edition 2005
264 1 _aTotowa, NJ
_bHumana Press
_c2005
300 _a1 recurso en línea (XVI, 364 páginas)
_b155 ilustraciones, 5 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v294
505 0 _aCell Migration -- Basic Cell Migration and Related Assays -- Boyden Chamber Assay -- Wound-Healing Assay -- Analysis of Cell Migration Using the Dunn Chemotaxis Chamber and Time-Lapse Microscopy -- Cell-Adhesion Assays -- Cell-Spreading Assays -- Cell-Scatter Assay -- Cell Migration Analyses Within Fibroblast-Derived 3-D Matrices -- Analysis of Cell Migration in Specialized Cell Systems -- Tumor Cell Invasion Assays -- Analysis of Endothelial Cell Migration Under Flow -- Angiogenesis Assays in the Chick CAM -- Investigations of Neuronal Migration in the Central Nervous System -- Analysis of Cell Migration in Model Organisms -- Analysis of Cell Migration in Caenorhabditis elegans -- Analysis of Cell Migration Using Drosophila as a Model System -- Imaging Cell Movement During Dorsal Closure in Drosophila Embryos -- Analysis of Cell Movements in Zebrafish Embryos -- The Xenopus Embryo as a Model System for Studies of Cell Migration -- Neural Crest Migration Methods in the Chicken Embryo -- In Vivo and In Vitro Models of Mammalian Angiogenesis -- Biochemical and Novel Approaches in Cell Migration -- Measurement of Protein Tyrosine Phosphorylation in Cell Adhesion -- Cell-Adhesion Assays -- Application of Microscope-Based FRET to Study Molecular Interactions in Focal Adhesions of Live Cells -- Use of Multiphoton Imaging for Studying Cell Migration in the Mouse -- Using Microfluidic Channel Networks to Generate Gradients for Studying Cell Migration.
520 _aCentral to the better understanding of both molecular mechanisms and disease, cell migration plays an essential role in a variety of biological processes and is now the subject of intense study using an array of powerful new technologies. In Cell Migration: Developmental Methods and Protocols, researchers describe in step-by-step detail their most successful techniques for studying the macromolecular machinery of cell movement. These readily reproducible protocols include a wide range of novel and state-of-the-art methodologies, as well as many classic methods, for use in cultured cells, different model organisms, and specialized cells in both normal development and disease. Highlights include basic assays that apply to all cell migration studies in vitro, assays in various model organisms, and assays for cancer cells, endothelial cells, and neurons both in vitro and in animal models. The authors also offer several novel approaches to the study of cell migration, as well as extensive coverage of cell migration studies in developmental and disease models. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Cell Migration: Developmental Methods and Protocols offers researchers easy access to many readily reproducible techniques for the optimally productive investigation of cell migration in today's interdisciplinary experimental environment.
988 _aSpringer_Protocols_2005
650 7 _2embne
_9165668
_aFisiología celular
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781617375323
776 0 8 _iPrinted edition:
_z9781588293824
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592598609
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b12/2023
_dz
_eb
_zSI