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020 _a9781493972654
024 7 _a10.1007/978-1-4939-7265-4
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH211
_b2017 EB
245 0 0 _aSuper-Resolution Microscopy :
_bMethods and Protocols
_cedited by Holger Erfle
250 _a1st edition 2017
264 1 _aNew York, NY
_bSpringer International Publishing
_c2017
300 _a1 recurso en línea (XI, 262 páginas)
_b48 ilustraciones, 37 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1663
505 0 _aSuper-Resolution Microscopy Techniques and their Potential for Applications in Radiation Biophysics -- Managing the Introduction of Super-Resolution Microscopy into a Core Facility -- Live-Cell STED Imaging with the HyPer2 Biosensor -- Diffraction-Unlimited Fluorescence Imaging with an EasySTED Retrofitted Confocal Microscope -- Two-Photon STED Microscopy for Nanoscale Imaging of Neural Morphology In Vivo -- STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells -- Four Channel Super-Resolution Imaging by 3-D Structured Illumination -- Correlative SIM-STORM Microscopy -- Correlative Super-Resolution Fluorescence Imaging and Atomic Force Microscopy for the Characterization of Biological Samples -- Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic Analysis of Fluorophore Blinking Cycles -- Two-Color Single-Molecule Tracking in Live Cells -- Fully Automated Targeted Confocal and Single Molecule Localization Microscopy -- Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution Microscopy -- Correlative In-Resin Super-Resolution Fluorescence and Electron Microscopy of Cultured Cells -- Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and their use in Cellular Imaging Experiments -- Measuring Nanometer Distances between Fluorescent Labels Step-by-Step -- Correlative Single Molecule Localization Microscopy and Confocal Microscopy -- Correlative Fluorescence Super-Resolution Localization Microscopy and Platinum Replica EM on Unroofed Cells -- In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT -- Super-Resolution High Content Screening and Analysis.
520 _aThis volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural morphology in vivo; correlative SIM-STORM microscopy; two-color single-molecule tracking in live cells; and correlative single molecule localization microscopy and confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Super-Resolution Microscopy: Methods and Protocols is a valuable resource for both established and novel researchers and users in this field.
988 _aSpringer_Protocols_2017
650 7 _2embne
_9677489
_aMicroscopios
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781493972647
776 0 8 _iPrinted edition:
_z9781493972661
776 0 8 _iPrinted edition:
_z9781493984350
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7265-4
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b12/2023
_dz
_eb
_zSI