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008			161201s2017 xxua o |||| 0|eng d
020			_a9781493965885
024	7		_a10.1007/978-1-4939-6588-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC669 _b2017 EB
245	0	0	_aCardiac Gene Therapy : _bMethods and Protocols _cedited by Kiyotake Ishikawa
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 355 páginas) _b93 ilustraciones, 78 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1521
505	0		_aCurrent Methods in Cardiac Gene Therapy: Overview -- Silencing Genes in the Heart -- Generation of Efficient miRNA Inhibitors Using Tough Decoy Constructs -- Efficient Genome Editing in Induced Pluripotent Stem Cells with Engineered Nucleases In Vitro -- Direct Cardiac Reprogramming as a Novel Therapeutic Strategy for Treatment of Myocardial Infarction -- Production and Characterization of Vectors Based on the Cardiotropic AAV Serotype 9 -- Cell-Based Measurement of Neutralizing Antibodies Against Adeno-Associated Virus (AAV) -- Synthesis of Modified mRNA for Myocardial Delivery -- Exosomes-Based Gene Therapy for MicroRNA Delivery -- Lipidoid mRNA Nanoparticles for Myocardial Delivery in Rodents -- Gene Transfer in Isolated Adult Cardiomyocytes -- Gene Transfer in Cardiomyocytes Derived from ES and iPS Cells -- Gene Transfer to Rodent Hearts In Vivo -- Ultrasound-Targeted Microbubble Destruction for Cardiac Gene Delivery -- A Needleless Liquid Jet Injection Delivery Approach for Cardiac Gene Therapy -- Cardiac Gene Delivery in Large Animal Models: Antegrade Techniques -- Direct Myocardial Injection of Vectors -- Selective Pressure-Regulate Retroinfusion for Gene Therapy Application in Ischemic Heart Disease -- Cardiac Gene Delivery Using Recirculating Devices -- Molecular Cardiac Surgery with Recirculating Delivery (MCARD): Procedure and Vector Transfer -- Gene Delivery for the Generation of Bioartificial Pacemaker -- Gene Therapy for Post-Infarction Ventricular Tachycardia -- MicroRNA Delivery Strategies to the Lung in a Model of Pulmonary Hypertension -- Inhaled Gene Transfer for Pulmonary Circulation.
520			_aThis detailed book provides methodological information on cardiac gene delivery, from classic to state-of-the-art technologies and techniques. Efficient, cardiac-specific, and safe vectors, as well as refined vector delivery methods, are key for successful cardiac gene transfer and eventually for improving patients' outcomes. Newer vectors and more efficient vector delivery methods have the potential to dramatically improve gene transduction efficacy, while novel gene manipulation techniques enforce the therapeutic power and broaden disease targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiac Gene Therapy: Methods and Protocols serves as a valuable tool for molecular biologists and physiologists in the cardiology field conducting cardiac gene transfer research, which will ultimately lead to further advancements in the vital field.
988			_aSpringer_Protocols_2017
650		7	_2embne _9169062 _aAparato circulatorio _xEnfermedades _xAspectos genéticos _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493965861
776	0	8	_iPrinted edition: _z9781493965878
776	0	8	_iPrinted edition: _z9781493982431
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6588-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI