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020 _a9781592599585
024 7 _a10.1385/1592599583
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aTP248.27 .Y43
_b2006 EB
245 0 0 _aYeast Protocols
_cedited by Wei Xiao
250 _a2nd edition 2006
264 1 _aTotowa, NJ
_bHumana Press
_c2006
300 _a1 recurso en línea (XII, 392 páginas)
_b104 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v313
505 0 _aBasic Investigations in Saccharomyces cerevisiae -- Isolation of Nucleic Acids -- Purification of Yeast Peroxisomes -- Isolation of Yeast Plasma Membranes -- Isolation of Yeast Mitochondria -- Extraction of Yeast Lipids -- Two-Dimensional Gel Electrophoresis of Total Yeast Proteins -- Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes -- Analysis of Yeast Lipids -- Yeast Fluorescence Microscopy -- Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombe -- Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method -- Mutagenesis -- Gene Disruption in the Budding Yeast Saccharomyces cerevisiae -- Inducible Degron and Its Application to Creating Conditional Mutants -- Synthetic Lethal Screen -- Synthetic Genetic Array Analysis in Saccharomyces cerevisiae -- Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates -- Chromatin Assembly in a Crude Fraction From Yeast Cells -- Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding Yeast -- Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome Positioning -- Study of Transcriptional Regulation Using a Reporter Gene Assay -- Assessing Telomeric Phenotypes -- Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast -- Production of Heterologous Proteins in Yeast With the Aid of the Hsp150? Carrier -- Yeast Two-Hybrid System Screening -- Import of Precursor Proteins Into Isolated Yeast Mitochondria.
520 _aIn this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred during the last decade to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research. Yeast Protocols, Second Edition contains 27 optimized and readily reproducible techniques suitable for both yeast researchers and investigators who wish to use yeast to study their favorite genes from other organisms. The authors provide detailed methods for the isolation of subcellular components, including organelles and macromolecules; for the basic cellular and molecular analysis specific for yeast cells; and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Yeast Protocols, Second Edition offers researchers a collection of traditional and novel protocols essential for studying yeasts, as well as genes from other organisms.
988 _aSpringer_Protocols_2006
650 7 _2embne
_9666009
_aLevaduras
_xBiotecnología
776 0 8 _iPrinted edition:
_z9781617375699
776 0 8 _iPrinted edition:
_z9781588294371
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592599583
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b12/2023
_dz
_eb
_zSI