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020 _a9781592590810
024 7 _a10.1385/1592590810
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aRB43.7
_b2001 EB
245 0 0 _aMolecular Pathology Protocols
_cedited by Anthony A. Killeen
250 _a1st edition 2001
264 1 _aTotowa, NJ
_bHumana Press
_c2001
300 _a1 recurso en línea (XIV, 491 páginas)
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Medicine
_x1940-6037
_v49
505 0 _aDNA Extraction from Paraffin-Embedded Tissues -- DNA Extraction from Fresh or Frozen Tissues -- RNA Extraction from Fresh or Frozen Tissues -- Single-Strand Conformation Polymorphism Analysis of Mutations in Exons 4-8 of the TP53 Gene -- Cleavase® Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection -- Detection of Telomerase by In Situ Hybridization and by the Polymerase Chain Reaction-Based Telomerase Activity Assay -- Detection of Microsatellite Instability -- Polymerase Chain Reaction Clonality Assays Based on X-Linked Genes -- Fluorescent In Situ Hybridization -- HER-2/neu Oncogene Amplification Determined by Fluorescence In Situ Hybridization -- A Nested Reverse Transcription-Polymerase Chain Reaction Assay to Detect BCR/abl -- Detection of t(15;17)(q24;q21), inv(16)/t(16;16)(p13;q22), and t(8;21)(q22;q22) Anomalies in Acute Myeloid Leukemias -- Detection of t(14; 18)(q32;q21)-Associated BCL-2/JH Gene Fusion in Non-Hodgkin Lymphoma -- Detection of Breast Cancer Cells Using Immunomagnetic Beads and Reverse Transcriptase Polymerase Chain Reaction -- Molecular Detection of Circulating Prostate Cancer Cells -- Methods to Detect Clonal Gene Rearrangements in Lymphomas and Leukemias -- Monitoring of Bone Marrow Transplant Engraftment -- Direct Molecular Diagnosis of Multiple Endocrine Neoplasia Type 1 -- Molecular Detection of Multiple Endocrine Neoplasia Type 2 -- Assay for Detecting the I1307K Susceptibility Allele within the Adenomatous Polyposis ColiGene -- Detection of Human Papillomaviruses by Polymerase Chain Reaction and In Situ Hybridization -- Molecular Methods for Detecting Epstein-Barr Virus (Part I) -- Molecular Methods for Detecting Epstein-Barr Virus (Part II) -- Molecular Methods for Detecting Epstein-Barr Virus (Part III) -- Molecular Detection of Kaposi's Sarcoma-Associated Herpesvirus/ Human Herpesvirus-8 -- Diagnostic Applications of Quantitative Polymerase Chain Reaction for Cytomegalovirus -- A Colorimetric Microtiter Plate Polymerase Chain Reaction System That Detects Herpes Simplex Virus in Cerebrospinal Fluid and Discriminates Genotypes 1 and 2 -- Detection and Typing of Hepatitis C Virus -- Detection and Speciation of Mycobacteria in Formalin-Fixed, Paraffin-Embedded Tissue Sections -- Ultrasensitive Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma by the AMPLICOR and COBAS AMPLICOR HIV-1 MONITOR™ Tests -- Molecular Diagnosis of Hereditary Thrombotic Disorders -- Prenatal Genotyping of the RhD Locus to Identify Fetuses at Risk for Hemolytic Disease of the Newborn -- Molecular Diagnosis of Hereditary Hemochromatosis -- Genotyping of Apolipoprotein E -- Genotyping for Functionally Important Human CYP2D6*4 (B) Mutation Using TaqMan Probes.
520 _aBecause molecular pathology has only recently emerged from the laboratory, many molecular pathology protocols are still to be found only in the primary literature. In Molecular Pathology Protocols, Anthony Killeen has assembled a collection of readily reproducible molecular pathology techniques that are either frequently performed or recognized for their significant diagnostic utility. Each method is described in step-by-step detail by a leading molecular pathologist or laboratory scientist who has developed it or used it extensively. These clinical laboratory techniques can be used for the diagnosis or monitoring of cancer, hematological malignancies, infectious diseases, and selected genetic disorders. Cutting-edge methods include telomerase, microsatellite instability, chromosomal translocations, and fluorescent in situ hybridization assays. Time-tested practices and numerous tips on how to avoid pitfalls ensure robust and successful results. Comprehensive and path-breaking, Molecular Pathology Protocols will enable clinical laboratories to introduce new molecular pathology tests and lay the groundwork for a much-needed standardization in this rapidly developing field.
988 _aSpringer_Protocols_2001
650 7 _2embne
_9158916
_aDiagnóstico molecular
776 0 8 _iPrinted edition:
_z9781617371318
776 0 8 _iPrinted edition:
_z9781489942227
776 0 8 _iPrinted edition:
_z9780896036819
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590810
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b12/2023
_dz
_eb
_zSI