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008			100301s1998 xxu| fo |||| 0|eng d
020			_a9781592592043
024	7		_a10.1385/0896034437 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRA1057.5 _b1998 EB
245	0	0	_aForensic DNA Profiling Protocols _cedited by Patrick J. Lincoln
250			_a1st edition 1998
264		1	_aTotowa, NJ _bHumana Press _c1998
300			_a1 recurso en línea (X, 309 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v98
505	0		_aRecovery of High-Molecular-Weight DNA from Blood and Forensic Specimens -- Recovery of DNA for PCR Amplification from Blood and Forensic Samples Using a Chelating Resin -- PCR Analysis of DNA from Fresh and Decomposed Bodies and Skeletal Remains in Medicolegal Death Investigations -- PCR Analysis from Cigaret Butts, Postage Stamps, Envelope Sealing Flaps, and Other Saliva-Stained Material -- Quantification of DNA By Slot-Blot Analysis -- Rapid Assessment of PCR Product Quality and Quantity by Capillary Electrophoresis -- Southern Blotting of Genomic DNA for DNA Profiling -- Preparation and Use of Alkaline-Phosphatase-Conjugated Oligonucleotide Probes for Single-Locus and Multilocus VNTR Analysis -- Preparation and Use of 32P-Labeled Single-Locus VNTR Probes in Identity Testing -- Statistical Methods Employed in Evaluation of Single-Locus Probe Results in Criminal Identity Cases -- Interpretation and Statistical Evaluation of Multilocus DNA Fingerprints in Paternity and Relationship Testing -- Overview of PCR-Based Systems in Identity Testing -- An Introduction to PCR Primer Design and Optimization of Amplification Reactions -- Analysis of Amplified Fragment-Length Polymorphisms (VNTR/STR Loci) for Human Identity Testing -- Amplification of Short Tandem Repeat Loci Using PCR -- Manual Electrophoretic Methods for Genotyping Amplified STR Loci -- 17 Genotyping STR Loci Using an Automated DNA Sequencer -- Separation of PCR Fragments by Means of Direct Blotting Electrophoresis -- 19 Amplification and Sequencing of Mitochondrial DNA in Forensic Casework -- Minisatellite Variant Repeat Unit Mapping Using PCR (MVR-PCR) -- Sex Determination by PCR Analysis of the X-Y Amelogenin Gene -- Species Determination by Analysis of the Cytochrome b Gene -- Use of the AmpliType PM + HLA DQA1 PCR Amplification and Typing Kits for Identity Testing -- The Use of Capillary Electrophoresis in Genotyping STR Loci -- Solid-Phase Minisequencing as a Tool to Detect DNA Polymorphism.
520			_aIn Forensic DNA Profiling Protocols, leading forensic experts from around the world describe in detail their time-proven methods for identity testing through DNA analysis. Their state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The book covers PCR-based test systems, the now widely used STR typing systems (complete with details of both manual and automated detection systems), repeat unit mapping (MVR), direct-phase minisequencing, capillary electrophoresis, and direct blotting. Also fully discussed are the recovery of DNA from a wide range of sample types (blood, semen, skeletal remains, and saliva), species testing, sex determination, and mitochondrial DNA testing. Forensic DNA Profiling Protocols captures and makes practical for all laboratories the whole array of new technologies and techniques that have revolutionized forensic science. The readily reproducible methods elucidated here represent the state-of-the-art for those scientists who want to introduce, establish, and perfect DNA identification techniques in their laboratories.
988			_aSpringer_Protocols_1998
650		7	_2embne _9461107 _aADN _xAnálisis
776	0	8	_iPrinted edition: _z9781617370380
776	0	8	_iPrinted edition: _z9780896034433
776	0	8	_iPrinted edition: _z9781489942562
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896034437 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b02/2024 _dz _ean _zSI