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007			cr nn 008mamaa
008			110810s2011 xxu| fo |||| 0|eng d
020			_a9781617791949
024	7		_a10.1007/978-1-61779-194-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR89.7 _b2011 EB
245	0	0	_aNitrogen Fixation : _bMethods and Protocols _cedited by Markus W. Ribbe
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (X, 326 páginas) _b72 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v766
505	0		_aHistoric Overview of Nitrogenase Research -- Mechanism of Mo-Dependent Nitrogenase -- Assembly of Nitrogenase MoFe Protein -- Genomic Analysis of Nitrogen Fixation -- Enzymatic Systems with Homology to Nitrogenase -- Molecular Biology and Genetic Engineering in Nitrogen Fixation -- Purification of Nitrogenase Proteins -- Assays of Nitrogenase Reaction Products -- Methods for Nitrogenase-Like Dark Operative Protochlorophyllide Oxidoreductase -- X-Ray Crystallography -- X-Ray Absorption Spectroscopy -- Small Angle X-Ray Scattering Spectroscopy -- Electron Paramagnetic Resonance Spectroscopy -- Magnetic Circular Dichroism Spectroscopy -- Mössbauer Spectroscopy -- Protocols for Cofactor Isoation of Nitrogenase -- Techniques for Functional and Structural Modeling of Nitrogenase -- Nitrogenase Structure and Function Relationships by Density Functional Theory -- Modeling the MoFe Nitrogenase System with Broken Symmetry Density Functional Theory.
520			_aFew problems in protein biochemistry have proven to be as challenging and recalcitrant as the molecular description of nitrogenase, the catalyst of one of the most remarkable chemical transformations in biological systems: the nucleotide-dependent reduction of atmospheric dinitrogen to bioavailable ammonia.  In Nitrogen Fixation: Methods and Protocols, recognized experts in the field provide an up-to-date, in-depth overview of the methods that have been applied to studying the nitrogenase at a molecular level, ranging from genetic, biochemical, spectroscopic, and chemical methods to theoretical calculations. In addition, techniques used to study an enzyme system that is homologous to nitrogenase are described in this book.  Written in the highly successful Methods in Molecular Biology™ series format, methods chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and cutting-edge, Nitrogen Fixation: Methods and Protocols will be useful for anyone interested in nitrogenase research and willing to venture further toward addressing the remaining mechanistic and biosynthetic questions of this fascinating enzyme system.
988			_aSpringer_Protocols_2011
650		7	_2embne _9182353 _aNitrógeno _xMetabolismo
776	0	8	_iPrinted edition: _z9781617791932
776	0	8	_iPrinted edition: _z9781617791956
776	0	8	_iPrinted edition: _z9781493958412
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-194-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b02/2024 _dz _ean _zSI