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008			111105s2012 xxu| fo |||| 0|eng d
020			_a9781617793677
024	7		_a10.1007/978-1-61779-367-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH585.2 _b2012 EB
245	0	0	_aHuman Cell Culture Protocols _cedited by Ragai R. Mitry, Robin D. Hughes
250			_a3rd edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XIV, 435 páginas) _b89 ilustraciones, 33 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v806
505	0		_aIntroduction to Cell Culture -- Isolation and Cultivation of Dermal Stem Cells that Differentiate into Functional Epidermal Melanocytes -- Isolation, Cultivation, and Application of Human Alveolar Epithelial Cells -- Culture of Parathyroid Cells -- Functional Analysis of Human Islets of Langerhans Maintained in Culture -- Conversion of Non-Endocrine Human Pancreatic Cells to Insulin Producing Cells for Treatment of Diabetes -- Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro -- Human Hepatocytes: Isolation, Culture, and Quality Procedures -- Preclinical Testing of Virotherapeutics for Primary and Secondary Tumors of the Liver -- Isolation and Functional Studies of Human Fetal Gastric Epithelium in Primary Culture -- Isolation, Characterization, and Culture of Normal Human Intestinal Crypt and Villus Cells -- Primary Culture of Human Renal Proximal Tubule Epithelial Cells and Interstitial Fibroblasts -- Glomerular Epithelial and Mesangial Cell Culture and Characterization -- Isolation and Culture of Human Adipocytes and Adipose Tissue -- Primary Culture of Human Adipocyte Precursor Cells: Expansion and Differentiation -- Primary Culture of Ovarian Cells for Research on Cell Interactions in the Hormonal Control of Steroidogenesis -- Human Vascular Smooth Muscle Cell Culture -- Culture of Human Endothelial Cells from Umbilical Veins -- Human Peripheral Blood Mononuclear Cell Culture for Flowcytometric Analysis of Phosphorylated Mitogen-Activated Protein Kinases -- Human CD4+CD25highCD127low/neg Regulatory T Cells -- Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation -- Isolation and Culture of Human Osteoblasts -- Human Osteoclast Culture and Phenotypic Characterization -- Effects of Temperature Generated from the Holmium:YAG Laser on Human Osteoblasts in Monolayer Tissue Culture -- Laser Microdissection Microscopy: Application to Cell Culture -- Automated Adherent Human Cell Culture (Mesenchymal Stem Cells) -- Culturing and Differentiating Human Mesenchymal Stem Cells for Biocompatible Scaffolds in Regenerative Medicine.
520			_aHuman cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement.  This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues.  With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research.
988			_aSpringer_Protocols_2012
650		7	_2embne _9139477 _aCitología
776	0	8	_iPrinted edition: _z9781617793660
776	0	8	_iPrinted edition: _z9781617793684
776	0	8	_iPrinted edition: _z9781493962082
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-367-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b02/2024 _dz _ean _zSI