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008			130217s2013 xxu| fo |||| 0|eng d
020			_a9781627033053
024	7		_a10.1007/978-1-62703-305-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2013 EB
245	0	0	_aProtein Acetylation : _bMethods and Protocols _cedited by Sandra B. Hake, Christian J. Janzen
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XI, 266 páginas) _b54 ilustraciones, 18 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029
505	0		_aValidation of Protein Acetylation by Mass Spectrometry -- Application of the CIRAD Mass Spectrometry Approach for Lysine Acetylation Site Discovery -- Application of the MIDAS Approach for Analysis of Lysine Acetylation Sites -- Application of High Content Biology to Yield Quantitative Spatial Proteomic Information on Protein Acetylations -- Towards the N-terminal Acetylome: An N-terminal Acetylated Peptide Enrichment Method Using CNBr-Activated Sepharose Resin -- Identification and Analysis of O-acetylated Sialoglycoproteins -- HPLC-based Quantification of in vitro N-terminal Acetylation -- Separation and Purification of Multiply Acetylated Proteins using Cation-exchange Chromatography -- In-gel N-acetylation for the Quantification of the Degree of Protein in vivo N-terminal Acetylation -- Computational Prediction of Lysine Acetylation Proteome-wide -- Generation and Characterization of Pan-specific Anti-acetyllysine Antibody -- Using Functional Proteome Microarrays to Study Protein Lysine Acetylation -- Quantitation of Nucleosome Acetylation and other Histone Post-Translational Modifications Using Microscale NU-ELISA -- Preparing Semisynthetic and Fully Synthetic Histones H3 and H4 to Modify the Nucleosome Core -- Production of Amino-terminally Acetylated Recombinant Proteins in E. coli -- Identification of Lysine Acetyltransferase Substrates using Bioorthogonal Chemical Proteomics -- Non-radioactive in-vitro Assays for Histone Deacetylases -- The Fluorescence-Based Acetylation Assay Using Thiol-Sensitive Probes -- Analysis of Protein Acetyltransferase Structure-function Relation by Surface-Enhanced Raman Scattering (SERS): A Tool to Screen and Characterize Small Molecule Modulators.
520			_aThousands of proteins have been identified to be acetylated. Immense research power has been dedicated to experiments to solve the biological implications of each and every protein acetylation. Two particular sites of protein acetylation have been described intensively: the N-terminal methionine residue of a nascent protein and lysine residues within a protein. In Protein Acetylation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study protein acetylation. These include methods and techniques for identification of protein acetylation, column- and gel electrophoresis-based approaches, computationally prediction,  and the biological response to protein acetylation. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Protein Acetylation: Methods and Protocols seeks to aid scientists in the further study of the technical aspects involved in understanding protein acetylation.
988			_aSpringer_Protocols_2013
650		7	_2embne _9139861 _aProteínas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781627033060
776	0	8	_iPrinted edition: _z9781627033046
776	0	8	_iPrinted edition: _z9781493959488
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-305-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b02/2024 _dz _ean _zSI