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008			120516s2012 xxu| fo |||| 0|eng d
020			_a9781617798399
024	7		_a10.1007/978-1-61779-839-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP623 _b2012 EB
245	0	0	_aRNA Abundance Analysis : _bMethods and Protocols _cedited by Hailing Jin, Walter Gassmann
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XIII, 231 páginas) _b22 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v883
505	0		_aSuperSAGE: Powerful Serial Analysis of Gene Expression -- Northern Blot Analysis for Expression Profiling of mRNAs and Small RNAs -- Construction of RNA-Seq Libraries from Large and Microscopic Tissues for the Illumina Sequencing Platform -- Strand-Specific RNA-Seq Applied to Malaria Samples -- RNA In Situ Hybridization in Arabidopsis -- Laser Microdissection of Cells and Isolation of High-Quality RNA After Cryosectioning -- Detection and Quantification of Alternative Splicing Variants Using RNA-Seq -- Separating and Analyzing Sulfur-Containing RNAs with Organomercury Gels -- RNAse Mapping and Quantitation of RNA Isoforms -- Detection and Quantification of Viral and Satellite RNAs in Plant Hosts -- In Situ Detection of Mature miRNAs in Plants Using LNA-Modified DNA Probes -- Small RNA Isolation and Library Construction for Expression Profiling of Small RNAs from Neurospora and Fusarium Using Illumina High Throughput Deep Sequencing -- Isolation and Profiling of Protein-Associated Small RNAs -- New Virus Discovery by Deep Sequencing of Small RNAs -- Global Assembly of Expressed Sequence Tags (ESTs) -- Computational Analysis of RNA-Seq -- Identification of MicroRNAs and Natural Antisense Transcript-Originated Endogenous siRNAs from Small-RNA Deep Sequencing Data.
520			_aRNA abundance analysis is one of the most important approaches for gene expression studies in the field of molecular biology. In RNA Abundance Analysis: Methods and Protocols, expert researchers cover a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches.  This volume includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, sulfur-containing RNAs, viral and satellite RNAs, RNA isoforms, and alternatively spliced RNA variants from various organisms, as well as key discussions of computational data processing for genome-wide datasets.  Written for the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Essential and easy to use, RNA Abundance Analysis: Methods and Protocols provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and modified RNAs, which can assist you in your gene expression studies.
988			_aSpringer_Protocols_2012
650		7	_2embne _9144940 _aARN
776	0	8	_iPrinted edition: _z9781617798382
776	0	8	_iPrinted edition: _z9781617798405
776	0	8	_iPrinted edition: _z9781493959167
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-839-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b02/2024 _dz _ean _zSI