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020 _a9781607613404
024 7 _a10.1007/978-1-60761-340-4
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP601
_b2009 EB
245 0 0 _aDNA Topoisomerases
_b: Methods and Protocols
_cedited by Duncan J. Clarke
250 _a1st edition 2009
264 1 _aTotowa, NJ
_bHumana Press
_c2009
300 _a1 recurso en línea (X, 268 páginas)
_b59 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v582
505 0 _a1. Introduction: Emerging Themes in DNA Topoisomerase Research -- 2. Analysis of DNA Topoisomers, Knots, and Catenanes by Agarose Gel Electrophoresis -- 3. Two-Dimensional Agarose Gel Electrophoresis of DNA Topoisomers -- 4. Cleavage of Plasmid DNA by Eukaryotic Topoisomerase II -- 5. Assays for the Preferential Binding of Human Topoisomerase I to Supercoiled DNA -- 6. Monitoring the Topoisomerase II DNA Gate Conformational Change with Fluorescence Resonance Energy Transfer -- 7. Single-Molecule Magnetic Tweezers Studies of Type IB Topoisomerases -- 8. Dissolution of Double Holliday Junctions by the Concerted Action of BLM and Topoisomerase III? -- 9. ChIP-on-Chip Analysis of DNA Topoisomerases -- 10. Analyzing Top2 Distribution on Yeast Chromosomes by Chromatin Immunoprecipitation -- 11. Binding of DNA Topoisomerases I and II to Replication Origins -- 12. Cytometric Assessment of DNA Damage Induced by DNA Topoisomerase Inhibitors -- 13. Analysis of the Topoisomerase II-Dependent Decatenation G2 Checkpoint and Checkpoint Kinases in Human Cells -- 14. Assaying Topoisomerase II Checkpoints in Yeast -- 15. Cytological Analysis of Chromosome Structural Defects that Result from Topoisomerase II Dysfunction -- 16. Top2 SUMO Conjugation in Yeast Cell Lysates -- 17. Analysis of SUMOylation of Topoisomerase II? with Xenopus Egg Extracts -- 18. The Dynamics of DNA Topoisomerase II? in Living Cells -- 19. Depletion and Mutation of Topoisomerase II in Animal Cells.
520 _aDue to their importance to cell biology, DNA topology-altering enzymes, collectively DNA topoisomerases and gyrases, have been studied thoroughly from a biochemical perspective, yet the expansion of research at the molecular and cellular levels continues to show a great deal of promise. In DNA Topoisomerases: Methods and Protocols, experts in the field present a compendium of protocols that extends into the new areas of research that have been recently uncovered in order to elucidate the methodology needed to study topoisomerases in the molecular and cellular context. The volume examines subjects such as the analysis of DNA topologies and the interaction of topoisomerases with DNA as tools to measure topoisomerase functions, the binding of topoisomerases to specific sites in the genomes of eukaryotes, including origins of DNA replication, as well as exploration of perturbed topoisomerase function. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes on trouble-shooting and avoiding known pitfalls. Cutting-edge and easy-to-use, DNA Topoisomerases: Methods and Protocols provides key content which reflects an expanded understanding of the functions of these essential enzymes in the hope of inspiring researchers to further important discoveries in this dynamic field.
988 _aSpringer_Protocols_2009
650 7 _2embne
_9138640
_aEnzimas
776 0 8 _iPrinted edition:
_z9781607613411
776 0 8 _iPrinted edition:
_z9781607613398
776 0 8 _iPrinted edition:
_z9781617796449
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-340-4
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b01/2024
_dz
_ean
_zSI