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020 _a9781592595624
024 7 _a10.1385/0896033554
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP702 .G577
_b1998 EB
245 0 0 _aGlycoanalysis Protocols
_cedited by Elizabeth F. Hounsell
250 _a2nd edition 1998
264 1 _aTotowa, NJ
_bHumana Press
_c1998
300 _a1 recurso en línea (X, 262 páginas)
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v76
505 0 _aCharacterization of Protein Glycosylation -- Analytical Methods for the Study of O-GlcNAc Glycoproteins and Glycopeptides -- Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography -- Exoglycosidase Sequencing of N-Linked Glycans by the Reagent Array Analysis Method (RAAM) -- HPAEC-PAD Analysis of Monosaccharides Released by Exoglycosidase Digestion Using the Carbopac MA1 Column -- HPLC and HPAEC of Oligosaccharides and Glycopeptides -- Analysis of N- and O-Glycans by Pyridylamination -- Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins -- Analysis of Glycosaminoglycans and Proteoglycans -- The Use of Cell and Organ Culture for the Study of Secreted Mucins -- Purification of Gastrointestinal Mucins and Analysis of Their O-Linked Oligosaccharides -- TLC in Structure and Recognition Studies of Glycosphingolipids -- Strategies for Preliminary Characterization of Novel Amphoteric Glycosphingolipids -- Analysis of the Carbohydrate and Lipid Components of Glycosylphosphatidylinos¡tol Structures -- Conformational Analysis of Biantennary Glycopeptides with a Resonance Energy Transfer Technique.
520 _aNow fully updated and considerably expanded, Glycoanalysis Protocols, 2nd ed., makes available to all protein scientists, and particularly those working with today's pharmaceuticals, the most advanced and reproducible glycoanalysis techniques currently in use. Developed by highly experienced carbohydrate chemists, biochemists, and physical chemists, these detailed, up-to-date, and proven analytical techniques cover the areas of glycoprotein macromolecular structural analysis, oligosaccharide profiling, lipid conjugate characterization, microorganism structure determination, and proteoglycan function. Special attention has been given to advanced analytical techniques in biotechnology during the production of recombinant glycoproteins and other therapeutics. Hailed as "indispensable" in its first edition, Glycoanalysis Protocols, 2nd ed., continues with vital, time-tested techniques addressing the needs of both biomedical researchers and protein macromolecular structural chemists. It will well serve all those starting work on the analysis of glycoproteins, as well as more experienced investigators seeking to augment their expertise.
988 _aSpringer_Protocols_1998
650 7 _2embne
_9138639
_aBioquímica
776 0 8 _iPrinted edition:
_z9781617370168
776 0 8 _iPrinted edition:
_z9780896033559
776 0 8 _iPrinted edition:
_z9781489942616
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033554
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b01/2024
_dz
_ean
_zSI