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999			_c393452 _d393452 _x1
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008			100301s2001 xxu| fo |||| 0|eng d
020			_a9781592590605
024	7		_a10.1385/1592590608 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552 .N36 _b2001 EB
245	0	0	_aNeurotrophin Protocols _cedited by Robert A. Rush
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (X, 274 páginas) _b41 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v169
505	0		_aNeurotrophin Quantification and Localization -- Localization of Neurotrophin Proteins Within the Central Nervous System by Immunohistochemistry -- Neurotrophin Immunohistochemistry in Peripheral Tissues -- Extraction and Quantification of the Neurotrophins -- Identifying Novel Proteins in Nervous Tissue Using Microsequencing Techniques -- RNA Quantification and Localization -- Quantification of mRNA Levels Using Northern Blotting -- Quantification of mRNA Levels Using Ribonuclease Protection Assay -- Quantification of Neurotrophin mRNA by RT-PCR -- Sensitive and Nonradioactive In Situ Detection of Neurotrophin mRNAs in the Nervous System -- Construction of Recombinant Proteins,and Transgenic Cells and Animals -- Expression of Neurotrophic Factors such as GDNF in the E. coli System -- Construction of Cells Expressing Neurotrophins -- Efficient Generation of Stable Pheochromocytoma (PC12) Cell Lines Using a Recombinant Retrovirus (LNC) -- Construction and Analysis of Transgenic Animals Overexpressing Neurotrophins -- Construction of Transgenic Animals Overproducing Neurotrophins and Their Receptors -- In Vivo Analysis -- Tracing with Radiolabeled Neurotrophins -- Toxin-Induced Death of Neurotrophin-Sensitive Neurons -- Oligonucleotides as Inhibitors of Protein Synthesis -- The IODO-GEN Method for Labeling and the Use of Retrograde Axonal Transport to Assay Neurotrophins -- Stereological Estimation of Neuronal Number The Optical Disector/Cavalieri Combination.
520			_aDespite the extraordinary growth of research interest in neurotrophic factors, the techniques available have often been inadequate or just emergent from other disciplines. In Neurotrophin Protocols, established leaders in the neurotrophin field detail their special expertise in a wide variety of key protein, RNA, recombinant, and in vivo techniques. The protocols range from immunological analysis for the cellular localization and quantification of the neurotrophins, to genetic manipulation of cells and animals for the analysis of biological function, to quantitative analysis of the active neurotrophin genes. There are also radiotracing techniques for studying neurotrophin transport in both the retrograde and anterograde directions, procedures for using immunotoxins to study the effects of eliminating a single class of neurons, and the essential stereological method for estimation of neuronal numbers. Each method includes not only detailed step-by-step instructions, but also a list of necessary equipment and supplies, and valuable notes spelling out quick tips and tricks of the trade. Timely and robust, Neurotrophin Protocols provides today's neuroscientists in both academia and industry with a comprehensive range of practical, readily reproducible methods for studying neurotrophins and the critically important effects they have on the nervous system.
988			_aSpringer_Protocols_2001
650		7	_2embne _9158907 _aNeurociencias
776	0	8	_iPrinted edition: _z9781489939838
776	0	8	_iPrinted edition: _z9781489939821
776	0	8	_iPrinted edition: _z9780896036994
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590608 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _ean _zSI