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008			100301s2004 xxu| fo |||| 0|eng d
020			_a9781592598120
024	7		_a10.1385/1592598129 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH671 _b2004 EB
245	0	0	_aApoptosis Methods and Protocols _cedited by Hugh J. M. Brady
250			_a1st edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XI, 304 páginas) _b74 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v282
505	0		_aMeasurement of Apoptosis by DNA Fragmentation -- Measurement of Caspase Activity in Cells Undergoing Apoptosis -- Flow Cytometry-Based Methods for Apoptosis Detection in Lymphoid Cells -- Methods of Analyzing Chromatin Changes Accompanying Apoptosis of Target Cells in Killer Cell Assays -- Detecting and Quantifying Apoptosis in Tissue Sections -- Immunoassay for Single-Stranded DNA in Apoptotic Cells -- Methods to Measure Membrane Potential and Permeability Transition in the Mitochondria During Apoptosis -- Measurement of Changes in Intracellular Calcium During Apoptosis -- Measurement of Changes in Apoptosis and Cell Cycle Regulatory Kinase Cdk2 -- Assays to Measure Stress-Activated MAPK Activity -- Methods for Culturing Primary Sympathetic Neurons and for Determining Neuronal Viability -- Immunocytochemical Techniques for Studying Apoptosis in Primary Sympathetic Neurons -- Measuring Programmed Cell Death in Plants -- Detection of Apoptosis in Drosophila -- Measurement of Apoptotic Cell Clearance In Vitro -- Yeast Two-Hybrid Screening for Proteins Interacting With the Anti-Apoptotic Protein A20 -- The Yeast Three-Hybrid System As a Tool to Study Caspases -- Cloning of Apoptosis-Related Genes by Representational Difference Analysis of cDNA -- Identification of Apoptosis Regulatory Genes Using Insertional Mutagenesis.
520			_aThe ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research in the 21st century. In Apoptosis Methods and Protocols, highly experienced researchers in the field of apoptosis describe in step-by-step detail the techniques they have perfected to investigate the critical steps involved in the apoptotic process. These readily reproducible methods range from flow cytometry and immunohistochemical procedures to kinase activity assays, yeast two-hybrid screening, and the cloning of novel genes by differential expression. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Apoptosis Methods and Protocols constitutes a key technical reference to the significant methodologies used in the field, as well as offering novice and experienced researchers alike powerful tools to illuminate the phenomenon of programmed cell death.
988			_aSpringer_Protocols_2004
650		7	_2embne _9139103 _aBiología molecular
776	0	8	_iPrinted edition: _z9781489939562
776	0	8	_iPrinted edition: _z9781489939555
776	0	8	_iPrinted edition: _z9780896038738
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592598129 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _ean _zSI