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008			100301s2004 xxu| fo |||| 0|eng d
020			_a9781592594344
024	7		_a10.1385/1592594344 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH600.15 _b2004 EB
245	0	0	_aMammalian Artificial Chromosomes _b: Methods and Protocols _cedited by Vittorio Sgaramella, Sandro Eridani
250			_a1st edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XI, 269 páginas) _b63 ilustraciones, 2 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v240
505	0		_aFrom Natural to Artificial Chromosomes -- Methodologies Applied to Domestic Animal Chromosomes -- Mapping of the Hotspots of Recombination in Human DNA Cloned as Yeast Artificial Chromosomes -- Genomic Imprinting and Its Effects on Genes and Chromosomes in Mammals -- Centromeres and Neocentromeres -- Identification of Start Sites of Bi-Directional DNA Synthesis at Eukaryotic DNA Replication Origins -- Telomere Length Analysis and In Vitro Telomerase Assay -- Telomerization of Mammalian Cells and Transplantation of Telomerized Cells in Immunodeficient Mice -- Chromosome-Based Vectors for Mammalian Cells -- Mammalian Artificial Chromosome Formation in Human Cells After Lipofection of a PAC Precursor -- Use of Natural and Artificial Chromosome Vectors for Animal Transgenesis -- Pronuclear Microinjection of Purified Artificial Chromosomes for Generation of Transgenic Mice -- Modification of Human Bacterial Artificial Chromosome Clones for Functional Studies and Therapeutic Applications.
520			_aAssembly of the basic units of mammalian chromosomes into artificial constructs, and the reduction of their components to more manageable dimensions, has now become a reality. In Mammalian Artificial Chromosomes: Methods and Protocols, leading scientists in cell biology, genetics, cell culturing, and gene transfer present their most productive methods for the preparation, characterization, and use of artificial chromosomes. The authors cover all relevant areas of artificial chromosome research-from basic genetics to daring attempts at building new gene therapy tools-and describe possible applications in genetic manipulation requiring large portions of the genome. Each fully tested protocol is described in step-by-step detail and includes a background introduction outlining the principle behind the technique, reagent and equipment lists, and tips on troubleshooting and how to avoid pitfalls. Cutting-edge and much-needed, Mammalian Artificial Chromosomes: Methods and Protocols offers a timely collection of meticulous techniques that will help elucidate the mechanisms of nuclear duplication, allow the introduction of changes into the human genome, and revolutionize our understanding of how chromosomes work.
988			_aSpringer_Protocols_2004
650		7	_2embne _9138064 _aMamíferos _xCitología
776	0	8	_iPrinted edition: _z9781489939487
776	0	8	_iPrinted edition: _z9781588290960
776	0	8	_iPrinted edition: _z9781489939470
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592594344 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _ean _zSI