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008			110103s2011 xxu| fo |||| 0|eng d
020			_a9781617379543
024	7		_a10.1007/978-1-61737-954-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRB155 _b2011 EB
245	0	0	_aDisease Gene Identification _b: Methods and Protocols _cedited by Johanna K. DiStefano
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XII, 312 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v700
505	0		_aTechnological Issues and Experimental Design of Gene Association Studies -- Statistical Issues in Gene Association Studies -- Identification of Causal Sequence Variants of Disease in the Next Generation Sequencing Era -- Microarray-Based Genome-Wide Association Studies Using Pooled DNA -- Medium-Throughput SNP Genotyping Using Mass Spectrometry: Multiplex SNP Genotyping Using the iPLEX® Gold Assay -- Targeted SNP Genotyping Using the TaqMan Assay -- Bar-Coded, Multiplexed Sequencing of Targeted DNA Regions Using the Illumina Genome Analyzer -- Site-Directed Mutagenesis -- Gene Expression Profiling of Tissues and Cell Lines: A Dual Color Microarray Method -- Methods for microRNA Microarray Profiling -- Allelic Expression Profiling to Dissect Genome-Wide Association Study Signals -- Quantitative Polymerase Chain Reaction: Quantitative PCR Using the Comparative Cq Method -- Genomic Analysis by Oligonucleotide Array Comparative Genomic Hybridization Utilizing Formalin-Fixed Paraffin Embedded Tissues -- RNA Mapping Protocols: Northern-Blot and Amplification of cDNA Ends -- High Content RNA Interference Assay: Analysis of Tau Hyperphosphorylation as a Generic Paradigm -- Integrative Systems Biology Approaches to Identify and Prioritize Disease and Drug Candidate Genes -- Identification of a Common Variant Affecting Human Episodic Memory Performance Using a Pooled Genome-Wide Association Approach: A Case Study of Disease Gene Identification -- RNAi-Based Functional Pharmacogenomics -- Genetic Predisposition to β-Thalassemia and Sickle Cell Anemia in Turkey: A Molecular Diagnostic Approach.
520			_aRecent efforts to characterize genetic variation in the human genome, coupled with the rapidly developing field of genomics, have lead directly to the development of new and innovative approaches to the identification of genes contributing to complex human diseases. In Disease Gene Identification: Methods and Protocols, expert researchers in the field provide up-to-date molecular methodologies used in the process of identifying a disease gene, from the initial stage of study design to the next stage of preliminary locus identification, and ending with stages involved in target characterization and validation. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and detailed tips on troubleshooting and avoiding known pitfalls. Authoritative and essential, Disease Gene Identification: Methods and Protocols seeks to aid scientists striving toward the identification and characterization of the many disease-related genes, which may someday pave the way for more accurate and improved methods of disease diagnosis as well as vital strategies for disease treatment and prevention.
988			_aSpringer_Protocols_2011
650		7	_2embne _9144879 _aGenética médica
776	0	8	_iPrinted edition: _z9781617379536
776	0	8	_iPrinted edition: _z9781617379550
776	0	8	_iPrinted edition: _z9781493961474
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61737-954-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _ean _zSI