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008			141020s2015 xxu| fo |||| 0|eng d
020			_a9781493916191
024	7		_a10.1007/978-1-4939-1619-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC931 .O73 _b2015 EB
245	0	0	_aOsteoporosis and Osteoarthritis _cedited by Jennifer J. Westendorf, Andre J. van Wijnen
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XI, 214 páginas) _b46 ilustraciones, 38 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1226
505	0		_aIsolation Of Osteocytes From Mature and Aged Murine Bone -- Primary Murine Growth Plate and Articular Chondrocyte Isolation and Culture -- Isolating Endosteal Mesenchymal Progenitors From Rodent Long Bones -- Engineering Cartilage Tissue By Pellet Co-Culture Of Chondrocytes And Mesenchymal Stem Cells. - Generation Of Induced Pluripotent Stem Cells -- Profiling Histone Modifications By Chromatin Immunoprecipitation Coupled To Deep Sequencing In Skeletal Cells -- Identification Of Micrornas In Human Plasma -- Immunohistochemistry Of Skeletal Tissues -- In Vivo Axial Loading Of The Mouse Tibia -- Four Point Bending Protocols To Study The Effects Of Dynamic Strain In Osteoblastic Cells In Vitro -- Epic-µct Imaging Of Articular Cartilage -- Mouse Models Of Osteoarthritis: Surgical Model Of Post-Traumatic Osteoarthritis Induced By Destabilization Of The Medial Meniscus -- Assessment Of Knee Joint Pain In Experimental Rodent Models Of Osteoarthritis -- Induction Of Fully Stabilized Cortical Bone Defects To Study Intramembranous Bone Regeneration -- Surgical Procedures And Experimental Outcomes Of Closed Fractures In Rodent Models                                                                                                                          .
520			_aIn Osteoporosis and Osteoarthritis expert investigators detail modern tools to enhance discovery in bone and cartilage biology and promote translational research in osteoporosis and osteoarthritis. New chapters describe methods to generate induced pluripotent stem cells, perform high-throughput chromatin analysis, assess loads on bone cells in vivo and in vitro, use state-of-the art imaging techniques to measure articular cartilage thickness and biochemical properties, and establish animal models of osteoarthritis and bone healing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Osteoporosis and Osteoarthritis shares successful techniques that can be applied to the study of either disease.
988			_aSpringer_Protocols_2015
650		7	_2embne _9141478 _aOsteoporosis
776	0	8	_iPrinted edition: _z9781493916207
776	0	8	_iPrinted edition: _z9781493916184
776	0	8	_iPrinted edition: _z9781493955275
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1619-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _ean _zSI