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020 _a9781592595327
024 7 _a10.1385/0896033082
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQR186.85
_b1995 EB
100 1 _aDavis, William C.
_c(Microbiologist)
_eautor
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
_9689799
245 1 0 _aMonoclonal Antibody Protocols
_cby William C. Davis
250 _a1st edition 1995
264 1 _aTotowa, NJ
_bHumana Press
_c1995
300 _a1 recurso en línea (X, 264 páginas)
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v45
505 0 _aMethods of Immunization to Enhance the Immune Response to Specific Antigens In Vivo in Preparation for Fusions Yielding Monoclonal Antibodies -- Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vitro -- Culture Conditions that Optimize Outgrowth of Hybridomas -- Production of Stable Heterohybridomas Producing Human Monoclonal Antibodies -- Production of Stable Bovine-Murine Interspecies Hybrids -- Production of Monoclonal Antibodies in Swine -- Production of Monoclonal Antibodies in Horses -- Use of Electric-Field-Mediated Cell Fusion to Produce Hybridomas Secreting Monoclonal Antibodies -- Rapid Isotyping of Mouse Monoclonal Antibodies -- Methods of Measuring Antibodies in Sera/Plasma, Ascites, and Tissue-Culture Media -- Measurement of Immunoglobulin Synthesis Using the ELISPOT Assay -- Use of Monoclonal Antibodies in Immunohistochemistry -- Use of Monoclonal Antibodies in Immunoelectron Microscopy -- Use of Monoclonal Antibodies for Western Blotting with Enhanced Chemiluminescent Detection -- The Use of Directly and Indirectly Labeled Monoclonal Antibodies in Flow Cytometry -- Use of Monoclonal Antibodies and Flow Cytometry to Cluster and Analyze Leukocyte Differentiation Molecules -- In Vivo and In Vitro Production of Monoclonal Antibodies -- Separation of Monoclonal Antibodies from Cell-Culture Supernatants and Ascites Fluid Using Thiophilic Agarose -- Detection, Purification, and Utilization of Murine Monoclonal IgM Antibodies -- Preparation and Use of Immunoaffinity Columns with Monoclonal Antibodies Without Purification from Ascites and Tissue-Culture Medium -- Antigen Purification by Monoclonal Antibody Immunoaffinity Chromatography -- Coupling of Monoclonal Antibodies with Fluorophores -- Coupling of Monoclonal Antibodies with Biotin -- Coupling of Monoclonal Antibodies with Enzymes -- Use of Monoclonal Antibodies with Magnetic Particles to Separate Cell Subpopulations by Negative Selection -- Use of Monoclonal Antibodies with Magnetic Particles to Separate Cell Subpopulations by Positive Selection.
520 _aSince the initial description of techniques to immortalize anti­ body-producing B-lymphocytes by fusion with tissue culture-adapted myeloma cells, methods have been developed to produce monoclonal antibodies of defined specificity in multiple animal species. Stable hybrids can be readily produced in mice using a number of myeloma and hybridoma cell lines. To obviate the problem of identifying fusion partners in other animal species, xenohybrids have been produced using B-lymphocytes from the relevant species and mouse myeloma cells. The use of xenohybrids has minimized the problem of obtain­ ing stable antibody-producing hybrids in all species examined thus far. Although alternative techniques are being developed to produce monoclonal antibodies by molecular methods, hybridoma technol­ ogy will remain the technology of choice for producing monoclonal antibodies for a variety of applications in research and industry. The objective of Monoclonal Antibody Protocols is to provide investigators with a set of methods for producing and using mono­ clonal antibodies in biomedical, agricultural, and biological sciences. The book is not intended to provide methodology for all possible applications, but rather a series of methods presented in an easy-- follow format that can be used by new and established investiga­ tors, graduate and postgraduate fellows, and technical staff.
988 _aSpringer_Protocols_1995
650 7 _2embne
_9405730
_aAnticuerpos monoclonales
776 0 8 _iPrinted edition:
_z9781489940773
776 0 8 _iPrinted edition:
_z9780896033085
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033082
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b01/2024
_dz
_ean
_zSI