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008			100715s2010 xxu| fo |||| 0|eng d
020			_a9781607612926
024	7		_a10.1007/978-1-60761-292-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP356.25 _b2010 EB
245	0	0	_aProtocols for Neural Cell Culture _cedited by Laurie C. Doering
250			_a4th edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (XII, 400 páginas) _b85 ilustraciones, 14 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aNeurosphere and Neural Colony-Forming Cell Assays -- Directed Neuronal Differentiation of Embryonic and Adult-Derived Neurosphere Cells -- Culture and Differentiation of Human Neural Stem Cells -- Neural Differentiation of Human Embryonic Stem Cells -- Isolation and Culture of Primary Human CNS Neural Cells -- Bioengineering Protocols for Neural Precursor Cell Expansion -- Intracellular Calcium Assays in Dissociated Primary Cortical Neurons -- Dissociated Hippocampal Cultures -- Primary Sensory and Motor Neuron Cultures -- Retinal Cell and Tissue Culture -- Preparation of Normal and Reactive Astrocyte Cultures -- Oligodendrocyte Progenitor Cell Culture -- Isolation of Microglia Subpopulations -- Microglia from Progenitor Cells in Mouse Neopallium -- Primary Schwann Cell Cultures -- Primary Dissociated Astrocyte and Neuron Co-culture -- Cerebellar Slice Cultures -- Hippocampal Slice Cultures -- Molecular Substrates for Growing Neurons in Culture -- Guidance and Outgrowth Assays for Embryonic Thalamic Axons -- Detection of Cell Death in Neuronal Cultures -- Live Imaging of Neural Cell Functions -- Tissue Culture Procedures and Tips.
520			_aSince the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies. The expert contributors to the fourth edition of Protocols for Neural Cell Culture refine existing protocols and present the emerging new techniques and culture media formulations linked with the many advances in neuroscience. While highlighting updated experimental procedures for many of the classical neural tissue culture preps, the volume addresses topics such as regenerative medicine and the methods to grow and expand embryonic and adult neural stem cells. Essential techniques for the isolation, expansion, and cryopreservation of neural tissue from mouse, rat, and human sources are covered, as well as methods to assess aspects of cell function (calcium imaging) and cell death. With its detailed, step-by-step laboratory protocols, this volume provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Authoritative and cutting-edge, Protocols for Neural Cell Culture, Fourth Edition continues the previous editions' tradition of exceptional quality and thorough coverage in tissue culture protocols for today's neuroscience in order to inspire researchers in the field to further explore this imperative area of study.
988			_aSpringer_Protocols_2010
650		7	_2embne _9672141 _aTejido nervioso _vCultivo y medios de cultivo
776	0	8	_iPrinted edition: _z9781607612933
776	0	8	_iPrinted edition: _z9781607612919
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-292-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _ean _zSI