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008			100301s2009 xxu| fo |||| 0|eng d
020			_a9781597455268
024	7		_a10.1007/978-1-59745-526-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP535 .K1 _b2009 EB
245	0	0	_aPotassium Channels _b: Methods and Protocols _cedited by Jonathan D. Lippiat
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (X, 302 páginas) _b59 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v491
505	0		_aMethods For Studying Potassium Channel Transcription And Mrna Splicing -- Identifying Transcriptional Regulatory Regions Using Reporter Genes and DNA-Protein Interactions by Chromatin Immunoprecipitation -- Quantitative RT-PCR Methods for Investigation of Low Copy Potassium Channel Gene Expression in Native Murine Arteries -- Cloning of Potassium Channel Splice Variants from Tissues and Cells -- Methods for Studying the Cell Biology of Potassium Channel Proteins -- Chemiluminescence Assays to Investigate Membrane Expression and Clathrin-Mediated Endocytosis of KATP Channels -- Investigation of KATP Channel Endocytosis by Immunofluorescence -- Investigation of KATP Channel Endocytosis and Cell Surface Density by Biotinylation and Western Blotting -- Lipid Microdomains and K+ Channel Compartmentation: Detergent and Non-Detergent-Based Methods for the Isolation and Characterisation of Cholesterol-Enriched Lipid Rafts -- Determination of Phosphoinositide Binding to K+ Channel Subunits Using a Protein-Lipid Overlay Assay -- Protein Complex Analysis of Native Brain Potassium Channels by Proteomics -- Electrophysiological Techniques For The Study Of Potassium Channel Function -- Xenopus Oocytes as a Heterologous Expression System for Studying Ion Channels with the Patch-Clamp Technique -- Whole-Cell Recording Using the Perforated Patch Clamp Technique -- Recording the Activity of ATP-Sensitive K+ Channels in Open-Cell Cell-Attached Configuration -- Planar Patch Clamp: Advances in Electrophysiology -- Analysing Steroid Modulation of BKCa Channels Reconstituted into Planar Lipid Bilayers -- Optical Techniques For The Study Of Potassium Channel Function And Interactions -- Using Bioluminescence Resonance Energy Transfer to Measure Ion Channel Assembly -- The Use of FRET Microscopy to Elucidate Steady State Channel Conformational Rearrangements and G Protein Interaction with the GIRK Channels -- The Voltage-Clamp Fluorometry Technique -- Methods For Studying Potassium Channelopathies And Pharmacological Modulators -- Identification of Mutations in the Kir6.2 Subunit of the KATP Channel -- Modulation of Potassium Ion Channel Proteins Utilising Antibodies -- Fluorescence-Based Tl+?Influx Assays as a Novel Approach for Characterization of Small-Conductance Ca2+?Activated K+ Channel Modulators -- Rubidium Efflux as a Tool for the Pharmacological Characterisation of Compounds with BK Channel Opening Properties -- Recording hERG Potassium Currents and Assessing the Effects of Compounds Using the Whole-Cell Patch-Clamp Technique.
520			_aDue to the importance of potassium channels in cellular function, researchers have been challenged to develop tools and experimental procedures to probe the function and identify chemicals that modulate the behaviour of this super-family of ion channels. In Potassium Channels: Methods and Protocols, experts in the field present a range of experimental approaches that have been developed to investigate potassium channel structure, function, pharmacology, cell biology, gene expression, and their role in disease. Many of these techniques study potassium channels as cellular proteins as well as the resultant membrane biophysics. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section, which highlights tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Potassium Channels: Methods and Protocols provides a wide range of approaches in order to aid current research projects and inspire new investigations.
988			_aSpringer_Protocols_2009
650		7	_2embne _9142266 _aMembranas celulares
776	0	8	_iPrinted edition: _z9781603279833
776	0	8	_iPrinted edition: _z9781617379406
776	0	8	_iPrinted edition: _z9781934115657
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-526-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b01/2024 _dz _ean _zSI