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008			100301s1999 xxu| fo |||| 0|eng d
020			_a9781592592586
024	7		_a10.1385/1592592589 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552 .C42 _b1999 EB
245	0	0	_aAdhesion Protein Protocols _cedited by Elisabetta Dejana, Monica Corada
250			_a1st edition 1999
264		1	_aTotowa, NJ _bHumana Press _c1999
300			_a1 recurso en línea (XIII, 229 páginas) _b4 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v96
505	0		_aMonoclonal Antibodies Specific for Leukocyte Adhesion Molecules -- Epitope Mapping -- Sequencing of Antibodies -- Purification Through Affinity Chromatography and Microsequencing -- Functional Analysis of Cell Adhesion Molecules -- Application of Polymerase Chain Reaction for the Discovery of New Adhesion Molecule Family Members -- Construction and Purification of Adhesion Molecule Immunoglobulin Chimeric Proteins -- Immunofluorescence of Cultured Cells -- Detection of Adhesion Molecules by Immunohistochemistry on Human and Murine Tissue Sections -- In situ Hybridization with RNA Probes -- Purification of Fibronectin from Human Plasma -- Adhesion to Matrix Proteins -- Leukocyte-Endothelial Monolayer Adhesion Assay (Static Conditions) -- Cell-Cell Adhesive Interactions in an In Vitro Flow Chamber -- Adhesion of Tumor Cells to Endothelium Under Static Conditions -- Adhesion of Tumor Cells Under Flow -- Platelet Adhesion to the Subendothelium Under Flow -- Leukocyte Transmigration Through Vascular Endothelium -- Cell Migration into a Wounded Area In Vitro -- Determination of the Endothelial Barrier Function In Vitro -- Induction of Intracellular Signals Through Binding of Adhesive Molecules -- Induction of Ca2+ Flux by Adhesion Molecules in Lymphocytes -- Integrin-Mediated Stimulation of Tyrosine Phosphorylation in Lymphoid Cells -- The Identification of Signaling Molecules by the Yeast Two-Hybrid System.
520			_aIn Adhesion Protein Protocols, an international corps of expert investigators describe their optimized techniques for both the identification of new cell adhesion proteins and the characterization of novel adhesive structures. Their cutting edge methods will enable both novice and experienced researchers readily to identify and clone new adhesion proteins, to successfully produce inhibitors of adhesive activity, to develop biological models for the assay of cell-to-matrix and cell-to-cell adhesion in vitro, and to measure the signaling activities of adhesion proteins. The protocols described here constitute a guide to some of the most important assays in cell biology, promising significant results not only to those interested in adhesion processes, but also to scientists from many different disciplines. Adhesion Protein Protocols is the first collection of methodologies devoted entirely to the study of adhesion molecules. By illuminating these molecules and the possibilities for manipulating them, the new experimental strategies collected here offer powerful approaches to the regulation of immunity, inflammation, tissue remodeling, and embryonic development. The book will become the standard source for those wishing to discover new adhesion molecules and to characterize their biological activity.
988			_aSpringer_Protocols_1999
650		7	_2embne _9139861 _aProteínas
650		7	_2embne _9139477 _aCitología
776	0	8	_iPrinted edition: _z9781489943187
776	0	8	_iPrinted edition: _z9781489943170
776	0	8	_iPrinted edition: _z9780896034174
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592589 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _ean _zSI