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020 _a9781493971251
024 7 _a10.1007/978-1-4939-7125-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH430
_b2017 EB
245 0 0 _aPlant Gene Regulatory Networks
_b: Methods and Protocols
_cedited by Kerstin Kaufmann, Bernd Mueller-Roeber
250 _a1st edition 2017
264 1 _aNew York, NY
_bSpringer International Publishing
_c2017
300 _a1 recurso en línea (XI, 350 páginas)
_b57 ilustraciones, 51 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1629
505 0 _aFrom Genes to Networks: Characterizing Gene-Regulatory Interactions in Plants -- Inducible Promoter Systems for Gene Perturbation Experiments in Arabidopsis -- Cell Type-Specific Gene Expression Profiling Using Fluorescence-Activated Nuclear Sorting -- Characterization of Cell Type-Specific DNA Binding Sites of Plant Transcription Factors using Chromatin Immunoprecipitation -- Yeast One and Two Hybrid High-Throughput Screenings using Arrayed Libraries -- SELEX-Seq: A Method to Determine DNA-Binding Specificities of Plant Transcription Factors -- Analysis of a Plant Transcriptional Regulatory Network using Transient Expression Systems -- Analysis of In Vivo Chromatin and Protein Interactions of Arabidopsis Transcript Elongation Factors -- Characterization of Mediator Complex and its Associated Proteins from Rice -- DNase I SIM: A Simplified In-Nucleus Method for DNase I Hypersensitive Site Sequencing -- In Situ Hi-C Library Preparation for Plants to Study their Three-Dimensional Chromatin Interactions on a Genome-Wide Scale -- Multiplexed Transcriptional Activation or Repression in Plants using CRISPR-dCas9 Based Systems -- Generation of dTALEs and Libraries of Synthetic TALE-Activated Promoters for Engineering of Gene Regulatory Networks in Plants -- Design of Knowledge Bases for Plant Gene Regulatory Networks -- AraNet: A Network Biology Server for Arabidopsis thaliana and Other Non-Model Plant Species -- Integration of Genome-Wide TF Binding and Gene Expression Data to Characterize Gene Regulatory Networks in Plant Development -- Predicting Transcription Factor-Binding Sites and their Cognate Transcription Factors using Gene Expression Data -- Computational Approaches to Study Gene Regulatory Networks -- Boolean Dynamic Modeling Approaches to Study Plant Gene Regulatory Networks: Integration, Validation and Prediction -- ODE-Based Modeling of Complex Regulatory Circuits -- Inferring Gene Regulatory Networks in the Arabidopsis Root using a Dynamic Bayesian Network Approach.
520 _aThis volume presents protocols that analyze and explore gene regulatory networks (GRNs) at different levels in plants. This book is divided into two parts: Part I introduces different experimental techniques used to study genes and their regulatory interactions in plants. Part II highlights different computational approaches used for the integration of experimental data and bioinformatics-based predictions of regulatory interactions. This part of the book also provides information on essential database resources that grant access to gene-regulatory and molecular interactions in different plant genomes, with a specific focus on Arabidopsis thaliana. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Gene Regulatory Networks: Methods and Protocols is a valuable resource for scientists and researchers interested in expanding their knowledge of GRNs.
988 _aSpringer_Protocols_2017
650 7 _2embne
_9138044
_aGenética
776 0 8 _iPrinted edition:
_z9781493971244
776 0 8 _iPrinted edition:
_z9781493971268
776 0 8 _iPrinted edition:
_z9781493983926
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7125-1
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b01/2024
_dz
_ean
_zSI