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008			101216s2011 xxu| fo |||| 0|eng d
020			_a9781617379642
024	7		_a10.1007/978-1-61737-964-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP535 .N1 _b2011 EB
245	0	0	_aNitric Oxide _b: Methods and Protocols _cedited by Helen O. McCarthy, Jonathan A. Coulter
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XI, 226 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v704
505	0		_aNitric Oxide Physiology and Pathology -- Ionizing Radiation-Induced DNA Strand Breaks and γ-H2AX Foci in Cells Exposed to Nitric Oxide -- Determination of S-Nitrosothiols in Biological Fluids by Chemiluminescence -- Measurement of Nitrite in Blood Samples Using the Ferricyanide-Based Hemoglobin Oxidation Assay -- Selective Fluorescent Activation for Bioimaging the Expression of Nitric Oxide in Cellular and In vivo Systems -- Real Time Measurement of Murine Hippocampus NO Levels in Response to Cerebral Ischemia/Reperfusion -- Detection of Low Levels of Nitric Oxide Using an Electrochemical Sensor -- Determination of the Scavenging Capacity Against Reactive Nitrogen Species by Automatic Flow Injection-Based Methodologies -- Aqueous Measurement of Nitric Oxide Using Membrane Inlet Mass Spectrometry -- Quantum Cascade Laser Technology for the Ultrasensitive Detection of Low Level Nitric Oxide -- Determination of In vivo Nitric Oxide Levels in Animal Tissues Using a Novel Spin Trapping Technology -- Beta Cell Protection by Inhibition of iNOS through Lentiviral Vector-Based Strategies -- Characterization of Nitric Oxide Delivery Systems Produced By Various Nanotechnologies -- Nitric Oxide Releasing Nanoparticle Synthesis and Characterization -- NOS Antagonism Using Viral Vectors as an Experimental Strategy: Implications for In vivo Studies of Cardio-Vascular Control and Peripheral Neuropathies.
520			_aThe past two decades have seen an explosion in the number of research articles relating to both the physiological and pathological responses evoked by nitric oxide generation. Despite this, accurate quantification of nitric oxide in either in vitro or in vivo models remains challenging, due to the relatively unstable nature of the molecule. In Nitric Oxide: Methods and Protocols, experts in the field provide a detailed reference manual packed with step-by-step approaches to reproduce various complex protocols within your own laboratory. Divided into two parts, the volume first covers various disparate protocols relating to the direct detection and quantification of nitric oxide, including techniques which detail how to image real time in vivo generation of nitric oxide, quantify nitric oxide production in the rat brain, and the detect ultralow levels of nitric oxide in the pM range, and the book then continues with a section on techniques designed to either inhibit or enhance nitric oxide, with an aim to achieve therapeutic gain, featuring the inhibition of the nitric oxide synthase enzymes using viral, shRNA delivery systems to prevent cardiovascular dysfunction, peripheral neuropathy, and graft rejection. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Nitric Oxide: Methods and Protocols examines numerous complex methodologies detailed in each chapter and proves to be an invaluable resource for anyone with an interest in nitric oxide research.
988			_aSpringer_Protocols_2011
650		7	_2embne _9673151 _aÓxido nitroso
776	0	8	_iPrinted edition: _z9781617379635
776	0	8	_iPrinted edition: _z9781617379659
776	0	8	_iPrinted edition: _z9781493957910
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61737-964-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _ean _zSI