000			04311nam a22003975i 4500
999			_c393381 _d393381 _x1
001			393381
003			ES-MaUEC
005			20240314174349.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			110414s2009 xxu| fo |||| 0|eng d
020			_a9781603273022
024	7		_a10.1007/978-1-60327-302-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR186.85 _b2009 EB
245	0	0	_aAntibody Phage Display _b: Methods and Protocols _cedited by Robert Aitken
250			_a2nd edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (X, 240 páginas) _b22 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v562
505	0		_aAntibody Phage Display: Overview of a Powerful Technology that Has Quickly Translated to the Clinic -- Design and Construction of Synthetic Phage-Displayed Fab Libraries -- Affinity Isolation of Antigen-Specific Circulating B Cells for Generation of Phage Display-Derived Human Monoclonal Antibodies -- Construction of Phage Antibody Repertoires from the Blood of West Nile Virus-Infected Donors -- Anti-?2GP-I and Anti-Prothrombin Antibodies Generated by Phage Display -- The Generation and Selection of Single-Domain, V Region Libraries from Nurse Sharks -- The Isolation of scFvs Against Small Target Molecules -- Display and Selection of scFv Antibodies on HEK-293T Cells -- Isolation of scFvs that Inhibit the NS3 Protease of Hepatitis C Virus by a Combination of Phage Display and a Bacterial Genetic Screen -- Guided Selection Methods Through Chain Shuffling -- Sequential Antigen Panning for Selection of Broadly Cross-Reactive HIV-1-Neutralizing Human Monoclonal Antibodies -- Selection of Antibodies Able to Rapidly Enter Mammalian Cells -- Identification of the Specificity of Isolated Phage Display Single-Chain Antibodies Using Yeast Two-Hybrid Screens -- Generation of Bispecific and Tandem Diabodies -- Intrabody Expression in Eukaryotic Cells -- High-Level Periplasmic Expression and Purificationof scFvs -- Expression of Single-Chain Fv Fragments in E. coli Cytoplasm -- High-Level Expression of a Phage Display-Derived scFv in Pichia pastoris.
520			_aSince its introduction almost 20 years ago, phage display technology has revolutionized approaches to the analysis of biomedical problems, quickly impacting the fields of immunology, cell biology, biotechnology, pharmacology, and drug discovery. In Antibody Phage Display: Methods and Protocols, Second Edition, expert researchers explore the latest in this cutting-edge technology, providing an invaluable resource that will guide readers in the design and execution of experiments based around antibody phage display. Chapters present a wide range of methods of isolating recombinant antibodies from phage display libraries, examine how the targets recognized by antibodies of interest can be identified, discuss the identification and exploitation of antibodies that can enter cells and bind to cytosolic targets, and include novel approaches to the expression of recombinant antibodies. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Detailed and innovative, Antibody Phage Display: Methods and Protocols, Second Edition is a critical handbook on phage display technology which is certain to stimulate the reader's imagination as much as it will guide future practice in the laboratory.
988			_aSpringer_Protocols_2009
650		7	_2embne _9671714 _aBacteriófagos
776	0	8	_iPrinted edition: _z9781607611158
776	0	8	_iPrinted edition: _z9781617794933
776	0	8	_iPrinted edition: _z9781603273015
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-302-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _ean _zSI