000			04681nam a22003975i 4500
999			_c393378 _d393378
001			393378
003			ES-MaUEC
005			20240213135611.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			111115s2012 xxu| fo |||| 0|eng d
020			_a9781617794124
024	7		_a10.1007/978-1-61779-412-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTA164 _b2012 EB
245	0	0	_aSynthetic Gene Networks _b: Methods and Protocols _cedited by Wilfried Weber, Martin Fussenegger.
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XI, 393 páginas) _b67 ilustraciones, 3 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v813
505	0		_aIn silico Implementation of Synthetic Gene Networks -- Standardization in Synthetic Biology -- Robust Optimal Design of Synthetic Biological Networks -- Predicting Synthetic Gene Networks -- Reprogramming a GFP Reporter Gene Subjects it to Complex Lentiviral Gene Regulation -- A High-throughput Microfluidic Method for Generating and Characterizing Transcription Factor Mutant Libraries -- Identifying and Optimizing Intracellular Protein-protein Interactions using Bacterial Genetic Selection -- Zinc-finger Nucleases Based Genome Engineering to Generate Isogenic Human Cell Lines -- RNA-based Networks: Using RNA Aptamers and Ribozymes as Synthetic Genetic Devices -- MicroRNA Circuits for Transcriptional Logic -- Light-regulated Gene Expression in Yeast -- Light-controlled Gene Switches in Mammalian Cells -- Expressed Protein Modifications: Making Synthetic Proteins -- Using Transcription Machinery Engineering to Elicit Complex Cellular Phenotypes -- Streamlining of a Pseudomonas putida Genome using a Combinatorial Deletion Method Based on Mini-transposon Insertion and the Flp-FRT Recombination System -- Transposon-based and Plasmid-based Genetic Tools for Editing Genomes of Gram-negative Bacteria -- Synthetic Networks: Oscillators and Toggle Switches for Escherichia coli -- Studying Microbial Communities in Biofilms -- Quantitative Analysis of the Spatiotemporal Dynamics of a Synthetic Predator-prey Ecosystem -- Drosophila S2 Schneider Cells - A Useful Tool for Rebuilding and Redesigning Approaches in Synthetic Biology -- Synthetic Gene Networks in Plant Systems -- Design and Construction of Synthetic Gene Networks in Mammalian Cells -- Synthetic Gene Networks as Blueprint for Smart Hydrogels.
520			_aThe rapid expansion of synthetic biology is due to the design and construction of synthetic gene networks that have opened many new avenues in fundamental and applied research. Synthetic Gene Networks: Methods and Protocols provides the necessary information to design and construct synthetic gene networks in different host backgrounds. Divided into four convenient sections, this volume focuses on design concepts to devise synthetic gene networks and how mathematical models can be applied to the predictable engineering of desired network features. The volume continues by highlighting the construction and validation of biologic tools, describing strategies to optimize and streamline the host cell for optimized network performance, and covering how optimally designed gene networks can be implemented in a large variety of host cells ranging from bacteria over yeast and insect cells to plant and mammalian cell culture. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Synthetic Gene Networks: Methods and Protocols serves as an invaluable resource for established biologists, engineers, and computer scientists or novices just entering into the rapidly growing field of synthetic biology.
988			_aSpringer_Protocols_2012
650		7	_2embne _9672737 _aBiología sintética
776	0	8	_iPrinted edition: _z9781617794117
776	0	8	_iPrinted edition: _z9781617794131
776	0	8	_iPrinted edition: _z9781493962242
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-412-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI