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008			160128s2016 xxu| fo |||| 0|eng d
020			_a9781493930432
024	7		_a10.1007/978-1-4939-3043-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR375 _b2016 EB
245	0	0	_aBaculovirus and Insect Cell Expression Protocols _cedited by David W. Murhammer.
250			_a3rd edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XII, 508 páginas) _b65 ilustraciones, 15 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1350
505	0		_aUseful Tips, Widely Used Techniques And Quantifying Cell Metabolic Behavior -- Introduction to Baculovirus Molecular Biology -- Baculovirus Transfer Vectors -- Recombinant Baculovirus Isolation -- Gene Expression In Mammalian Cells Using Bacmam, A Modified Baculovirus System -- Available Lepidopteran Insect Cell Lines -- Lepidopteran Insect Cell Line Isolation from Insect Tissue -- Development Of Serum-Free Media For Lepidopteran Insect Cell Lines -- Routine Maintenance And Storage Of Lepidopteran Insect Cell -- Small-Scale Production Of Recombinant Proteins Using The Baculovirus Expression Vector System -- Recombinant Protein Production In Large Scale Agitated Bioreactors Using The Baculovirus Expression Vector System -- Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors -- Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae -- Production Of Virus-Like Particles For Vaccination -- Alternative Strategies for Expressing Multi-Component Protein Complexes in Insect Cells -- Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression -- Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression -- Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression -- Introduction To The Use Of Baculoviruses As Biological Insecticides -- Baculovirus Insecticide Production In Insect Larvae -- Evaluation Of The Insecticidal Efficacy Of Wild Type And Recombinant Baculoviruses -- Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants -- Tubular Bioreactor for Probing Baculovirus Infection and Protein Production -- Gene Silencing In Insect Cells Using Rnai -- Using the Baculovirus/Insect Cell System to Study Apoptosis -- Generation of Envelope-Modified Baculoviruses for Gene Delivery into Mammalian Cells.
520			_aThe third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.
988			_aSpringer_Protocols_2016
650		7	_2embne _9413113 _aVirología
776	0	8	_iPrinted edition: _z9781493930425
776	0	8	_iPrinted edition: _z9781493930449
776	0	8	_iPrinted edition: _z9781493979783
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3043-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI