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008			100301s2005 xxu| fo |||| 0|eng d
020			_a9781592598380
024	7		_a10.1385/1592598382 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH585.2 _b2005 EB
245	0	0	_aBasic Cell Culture Protocols _cedited by Cheryl D. Helgason, Cindy L. Miller.
250			_a3rd edition 2005
264		1	_aTotowa, NJ _bHumana Press _c2005
300			_a1 recurso en línea (XII, 371 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v290
505	0		_aCulture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line -- Detection of Mycoplasma Contaminations -- Eradication of Mycoplasma Contaminations -- Authentication of Scientific Human Cell Lines -- Cytogenetic Analysis of Cell Lines -- Human and Mouse Hematopoietic Colony-Forming Cell Assays -- Isolation and Culture of Murine Macrophages -- Isolation and Culture of Human Macrophages -- Development of T-Lymphocytes in Mouse Fetal Thymus Organ Culture -- In Vitro Generation of Lymphocytes From Embryonic Stem Cells -- Hematopoietic Development of Human Embryonic Stem Cells in Culture -- Generation of Murine Stromal Cell Lines -- Culture of Human and Mouse Mesenchymal Cells -- Isolation, Purification, and Cultivation of Murine and Human Keratinocytes -- Isolation and Culture of Primary Human Hepatocytes -- Primary Kidney Proximal Tubule Cells -- Enzymatic Dissociation and Culture of Normal Human Mammary Tissue to Detect Progenitor Activity -- Generation and Differentiation of Neurospheres From Murine Embryonic Day 14 Central Nervous System Tissue -- Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells -- Adult Ventricular Cardiomyocytes -- Isolation and Culture of Primary Endothelial Cells -- Studying Leukocyte Rolling and Adhesion In Vitro Under Flow Conditions -- Isolation and Characterization of Side Population Cells -- Scalable Production of Embryonic Stem Cell-Derived Cells.
520			_aIn this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of the major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis). The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand today.
988			_aSpringer_Protocols_2005
650		7	_2embne _9139475 _aCélulas _vCultivo
776	0	8	_iPrinted edition: _z9781627039307
776	0	8	_iPrinted edition: _z9781588292841
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592598382 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI