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008			161130s2017 xxu| fo |||| 0|eng d
020			_a9781493966912
024	7		_a10.1007/978-1-4939-6691-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH447 _b2017 EB
245	0	0	_aMetagenomics _b: Methods and Protocols _cedited by Wolfgang R. Streit, Rolf Daniel.
250			_a2nd edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 303 páginas) _b48 ilustraciones, 24 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1539
505	0		_aConstruction of Small-Insert and Large-Insert Metagenomic Libraries -- Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies -- Construction and Screening Marine Metagenomic Large Insert Libraries -- Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment -- DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms -- Assessing Bacterial and Fungal Diversity in the Plant's Endosphere -- Shotgun Metagenomic Sequencing analysis of Soft-Rot Enterobacteriaceae in Polymicrobial Communities -- Cloning and Expression of Metagenomic DNA in Streptomyces lividans and its Subsequent Fermentation for Optimized Production -- Degradation Network Reconstruction Guided by Metagenomic Data -- Novel Tools for the Functional Expression of Metagenomics DNA -- A Microtiter Plate-Based Assay to Screen for Active and Stereoselective Hydrolytic Enzymes in Enzyme Libraries -- Screening for Cellulase Encoding Clones in Metagenomics Libraries -- Liquid Phase Multiplex High-Throughput Screening of Metagenomic Libraries using p-Nitrophenyl-Linked Substrates for Accessory Lignocellulosic Enzymes -- Screening Glycosyltransferases for Polyphenol Modifications -- Methods for the Isolation of Genes Encoding Novel PHA Matabolism Enzymes from Complex Microbial Communities -- Function-Based Metagenomic Library Screening and Heterologous Expression Strategy for Genes Encoding Phosphatase Activity -- Activity-Based Screening of Metagenomics Libraries for Hydrogenase Enzymes -- Screening for N-AHSL-Based-Signaling Interfering Enzymes -- Mining Microbial Signals for Enhanced Biodiscovery of Secondary Metabolites.
520			_aThis second edition explores up-to-date tools in various function-based technologies currently used in metagenomics. The chapters in this book discuss all of the working steps involved in these technologies, such as: DNA isolation from soils and marine samples followed by the construction and screening of libraries for diverse enzymes and biomolecules; current methods used to isolate DNA and construct large- and small-insert libraries from terrestrial and marine habitats; methods for establishing metagenome libraries in none-E.coli hosts; new molecular tools used for function-driven mining of metagenomic DNA; and screening protocols for a wide array of different genes encoding enzymes with relevance to biotechnology and ecology. Metagenomics: Methods and Protocols, Second Edition also provides detailed screening protocols for phosphatases, poly-hydroxyalkanoate, metabolism-related enzymes, stereoselective hydrolases, and microbial signals for the discovery of secondary metabolites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Metagenomics: Methods and Protocols, Second Edition is a collection of up-to-date metagenome protocols and tools for the recovery of numerous major types of biocatalysts, and allows researchers to easily setup these screens in any microbiology laboratory. .
988			_aSpringer_Protocols_2017
650		7	_2embne _9140738 _aGenética microbiana
776	0	8	_iPrinted edition: _z9781493966899
776	0	8	_iPrinted edition: _z9781493966905
776	0	8	_iPrinted edition: _z9781493982745
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6691-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI