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008			100301s2004 xxu| fo |||| 0|eng d
020			_a9781592597529
024	7		_a10.1385/1592597521 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH600.15 _b2004 EB
245	0	0	_aBacterial Artificial Chromosomes _b: Volume 1 Library Construction, Physical Mapping, and Sequencing _cedited by Shaying Zhao, Marvin Stodolsky.
250			_a1st edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XX, 360 páginas) _b71 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v255
505	0		_aBAC Library Construction -- Construction of Small Genome BAC Library for Functional and Genomic Applications -- Preparation of BAC Libraries From Bacterial Genomes by In Vitro Packaging -- Exploring Transformation-Associated Recombination Cloning for Selective Isolation of Genomic Regions -- Purification of BAC DNA -- Hybridization-Based Selection of BAC Clones -- Applications of Interspersed Repeat Sequence Polymerase Chain Reaction -- BAC Mapping Using Fluorescence In Situ Hybridization -- High-Throughput BAC Fingerprinting -- BAC End Sequencing -- Radiation Hybrid Mapping With BAC Ends -- Shotgun Library Construction for DNA Sequencing -- Rolling Circle Amplification for Sequencing Templates -- Transposon-Mediated Sequencing -- Pyrosequencing -- Use of Fimers to Eliminate Polymerase Chain Reaction and Primer-Dimer Artifacts and to Increase Yield in BAC-Sequencing Reactions -- Retrofitting BACs and PACs With LoxP Transposons to Generate Nested Deletions -- Preparing Nested-Deletion Template DNA for Field Inversion Gel Electrophoresis Analyses and Position-Specific End Sequencing With Transposon Primers -- BAC Finishing Strategies -- Using the TIGR Assembler in Shotgun Sequencing Projects -- Finishing "Working Draft" BAC Projects by Directed Sequencing With ThermoFidelase and Fimers -- Optimized Multiplex Polymerase Chain Reaction -- Assembly of DNA Sequencing Data -- Sequence Finishing -- Quality Assessment of Finished BAC Sequences.
520			_aThe ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1: Library Construction, Physical Mapping, and Sequencing presents readily reproducible techniques for library construction, physical mapping, and sequencing. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection of highly productive protocols that will prove useful to many bioscientists, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of specific genomes.
988			_aSpringer_Protocols_2004
650		7	_2embne _9139992 _aCromosomas
776	0	8	_iPrinted edition: _z9781617372889
776	0	8	_iPrinted edition: _z9780896039889
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592597521 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI