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008			170302s2017 xxu| fo |||| 0|eng d
020			_a9781493968572
024	7		_a10.1007/978-1-4939-6857-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR186.7 _b2017 EB
245	0	0	_aSynthetic Antibodies _b: Methods and Protocols _cedited by Thomas Tiller.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XIV, 420 páginas) _b102 ilustraciones, 74 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1575
505	0		_aAntibody Mimetics, Peptides, and Peptidomimetics -- Construction of a scFv Library with Synthetic, Non-Combinatorial CDR Diversity -- Enzymatic Assembly for scFv Library Construction -- Directed Evolution of Protein Thermal Stability Using Yeast Surface Display -- Whole Cell Panning with Phage Display -- Generating Conformation and Complex-Specific Synthetic Antibodies -- High-Throughput IgG Conversion of Phage Displayed Fab Antibody Fragments by AmplYFast -- Utilization of Selenocysteine for Site-Specific Antibody Conjugation -- Solubility Characterization and Imaging of Intrabodies Using GFP-Fusions -- Antibody Validation by Immunoprecipitation Followed by Mass Spectrometry Analysis -- Novel HPLC-Based Screening Method to Assess Developability of Antibody-Like Molecules -- Glycosylation Profiling of α/β T Cell Receptor Constant Domains Expressed in Mammalian Cells -- A Proximity-Based Assay for Identification of Ligand and Membrane Protein Interaction in Living Cells -- A Biotin Ligase-Based Assay for the Quantification of the Cytosolic Delivery of Therapeutic Proteins -- Data-Driven Antibody Engineering Using Genedata Biologics™ -- Selection of Aptamers Against Whole Living Cells: From Cell-SELEX to Identification of Biomarkers -- Rapid Selection of RNA Aptamers that Activate Fluorescence of Small Molecules -- An Enzyme-Linked Aptamer Sorbent Assay to Evaluate Aptamer Binding -- Incorporating Aptamers in the Multiple Analyte Profiling Assays (xMAP): Detection of C-Reactive Protein -- Transferring the Selectivity of a Natural Antibody into a Molecularly Imprinted Polymer -- Preparation of Molecularly Imprinted Microspheres by Precipitation Polymerization -- Generation of Janus Molecularly Imprinted Polymer Particles -- Surface Engineering of Nanoparticles to Create Synthetic Antibodies -- H5N1 Virus Plastic Antibody Based on Molecularly Imprinted Polymers -- Replacement of Antibodies in Pseudo-ELISAs: Molecularly Imprinted Nanoparticles for Vancomycin Detection -- Cell and Tissue Imaging with Molecularly Imprinted Polymers.
520			_aThis detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs). Part I includes methods that deal with amino-acid based synthetic antibodies. Brief protocols about the generation of antibody libraries are detailed, as well as techniques for antibody selection, characterization, and validation. This section is completed by a brief description of a bioinformatics platform that supports antibody engineering during research and development. Part II contains basic procedures about the selection and characterization of aptamer molecules, and Part III describes fundamental processes of MIP generation and application. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synthetic Antibodies: Methods and Protocols is an ideal guide for scientists seeking to propel the vital study of antibody research.
988			_aSpringer_Protocols_2017
650		7	_2embne _9143641 _aInmunoglobulinas
776	0	8	_iPrinted edition: _z9781493968558
776	0	8	_iPrinted edition: _z9781493968565
776	0	8	_iPrinted edition: _z9781493983193
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6857-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI