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008			100301s2006 xxu| fo |||| 0|eng d
020			_a9781597451581
024	7		_a10.1385/1597451584 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH442.2 _b2006 EB
245	0	0	_aYAC Protocols _cedited by Alasdair MacKenzie.
250			_a2nd edition 2006
264		1	_aTotowa, NJ _bHumana Press _c2006
300			_a1 recurso en línea (XVI, 204 páginas) _b49 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v349
505	0		_aMarkers, Selection, and Media in Yeast Artificial Chromosome Cloning -- Construction of Yeast Artificial Chromosome Libraries From Pathogens and Nonmodel Organisms -- Analysis and Screening of Yeast Artificial Chromosome Libraries by Filter Hybridization -- Analysis of Yeast Artificial Chromosome DNA by Restriction Digestion, Southern Blotting Nucleic Acid Hybridization, and Polymerase Chain Reaction -- Postgenomic Bioinformatic Analysis of Yeast Artificial Chromosome Sequence -- Transformation of Yeast Using Bioactive Beads With Surface-Immobilized Yeast Artificial Chromosomes -- Modification and Amplification of Yeast Artificial Chromosomes -- Modifying Yeast Artificial Chromosomes to Generate Cre/LoxP and FLP/FRT Site-Specific Deletions and Inversions -- Selective Isolation of Large Chromosomal Regions by Transformation-Associated Recombination Cloning for Structural and Functional Analysis of Mammalian Genomes -- A Polymerase Chain Reaction-Mediated Yeast Artificial Chromosome-Splitting Technology for Generating Targeted Yeast Artificial Chromosomes Subclones -- Genomic Reconstruction by Serial Mitotic Recombination of Yeast Artificial Chromosomes -- Targeting Vector Construction by Yeast Artificial Chromosome Modification -- Production of Yeast Artificial Chromosome Transgenic Mice by Pronuclear Injection of One-Cell Embryos -- Generation of Yeast Artificial Chromosome Transgenic Mice by Intracytoplasmic Sperm Injection -- Establishment of Cell Lines That Exhibit Correct Ontogenic Stage-Specific Gene Expression Profiles From Tissues of Yeast Artificial Chromosome Transgenic Mice Using Chemically Induced Growth Signals -- Application of Yeast Artificial Chromosomes in Fluorescence In Situ Hybridization -- Contribution of Yeast Artificial Chromosome-Based Physical Maps to the Final Assembly of the Trypanosoma cruzi Genome.
520			_aIt is now 10 years since the first edition of YAC Protocols was published in 1996. YAC Protocols was first produced to address the huge demand within the research community for a lab-based text that described in detail the wide range of uses for large insert yeast artificial chromosome (YAC) DNA clones. In doing this, the original editor, David Markie, and the many different contri- tors who provided descriptions of the protocols they used and developed, did a magnificent job. Indeed many of the techniques described within the first e- tion require little change and have stood up admirably to the test of time. Since the first edition, the use of YACs has proved invaluable for addressing a wide range of new biological problems ranging from those of basic biochemistry to assisting in the mapping and sequencing of the human genome. The requirement for a second edition of YAC Protocols was prompted by a number of major advances in biology since the publication of the first edition. These advances have included the sequencing of the human genome, and the genomes of a wide variety of other organisms, and the increased use of transgenic animals for understanding the molecular basis of human and animal disease.
988			_aSpringer_Protocols_2006
650		7	_2embne _9138683 _aHongos
650		7	_2embne _9141520 _aIngeniería genética
776	0	8	_iPrinted edition: _z9781617376733
776	0	8	_iPrinted edition: _z9781588296122
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1597451584 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI