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008			140823s2014 xxu| fo |||| 0|eng d
020			_a9781493912124
024	7		_a10.1007/978-1-4939-1212-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR185.8 .T24 _b2014 EB
245	0	0	_aT-Helper Cells _b: Methods and Protocols _cedited by Ari Waisman, Burkhard Becher.
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XI, 229 páginas) _b38 ilustraciones, 24 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1193
505	0		_aIsolation of T Cells from the Skin -- Isolation of Leukocytes from Mouse Central Nervous System -- Isolation of T Cells from the Gut -- T Cell Isolation from Mouse Kidneys -- Intracellular Staining for Cytokines and Transcription Factors -- Surface Capture Staining -- Mass Cytometry Analysis of Human T Cell Phenotype and Function -- Detection of NF-κB Pathway Activation in T Helper Cells -- Assessing the Suppressive Activity of Foxp3+ Regulatory T Cells -- In vitro Generation of Microbe Specific Human Th17 Cells -- In vitro Polarization of T-Helper Cells -- A Method for the Generation of TCR Retrogenic Mice -- Mouse Models of Allergic Airway Disease -- Induction of Colitis in Mice (T-Cell Transfer Model) -- Manipulation of T Cell Function Conditional Gene Targeting in T Cells -- Aldara-Induced Psoriasis-Like Skin Inflammation: Isolation and Characterization of Cutaneous Dendritic Cells and Innate Lymphocytes -- Induction of Passive EAE Using Myelin-Reactive CD4+ T Cells -- Experimental Mouse Models of T Cell-Dependent Inflammatory Bowel Disease -- Analysis of Chromosomal Aberrations in Murine HCC.
520			_aT-Helper Cells: Methods and Protocols presents a broad selection of cutting edge protocols that will enable the reader to capture the unique features of TH cells with tools developed for the isolation of TH cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo and in vivo. Chapters cover methods of isolating T cells from various tissues in mice, protocols for the analysis of T cell function and phenotype using various cutting edge technologies, methods allowing for the manipulation of T cell function in vitro and in vivo, and in vivo models of diseases in which T cells play a central role in the pathogenesis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, T-Helper Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the study of this amazingly versatile and potent cell type.
988			_aSpringer_Protocols_2014
650		7	_2embne _9139475 _aCélulas
650		7	_2embne _9138330 _aInmunología
776	0	8	_iPrinted edition: _z9781493912131
776	0	8	_iPrinted edition: _z9781493912117
776	0	8	_iPrinted edition: _z9781493946457
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1212-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI