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020 _a9781607618201
024 7 _a10.1007/978-1-60761-820-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQR47
_b2010 EB
245 0 0 _aOral Biology
_b: Molecular Techniques and Applications
_cedited by Gregory J. Seymour, Mary P. Cullinan, Nicholas C.K. Heng.
250 _a1st edition 2010
264 1 _aTotowa, NJ
_bHumana Press
_c2010
300 _a1 recurso en línea (XII, 406 páginas)
_b52 ilustraciones, 2 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v666
505 0 _aSaliva Studies -- Gene Therapy of Salivary Diseases -- Collection, Storage, and Processing of Saliva Samples for Downstream Molecular Applications -- Proteomic Analysis of Saliva: 2D Gel Electrophoresis, LC-MS/MS, and Western Blotting -- Transcriptomic Analyses of Saliva -- Oral Microbiology -- The Oral Microbiota: General Overview, Taxonomy, and Nucleic Acid Techniques -- Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE) -- Protocols to Study the Physiology of Oral Biofilms -- Adhesion of Yeast and Bacteria to Oral Surfaces -- Quantitative Analysis of Periodontal Pathogens by ELISA and Real-Time Polymerase Chain Reaction -- Bacterial Viability Determination in a Dentinal Tubule Infection Model by Confocal Laser Scanning Microscopy -- Characterization of Anti-competitor Activities Produced by Oral Bacteria -- Natural Transformation of Oral Streptococci -- Use of In Vivo-Induced Antigen Technology (IVIAT) to Identify Virulence Factors of Porphyromonas gingivalis -- Oral Bacterial Genome Sequencing Using the High-Throughput Roche Genome Sequencer FLX System -- Use of a Yeast-Based Membrane Protein Expression Technology to Overexpress Drug Resistance Efflux Pumps -- Cells and Tissues -- Explant Culture of Embryonic Craniofacial Tissues: Analyzing Effects of Signaling Molecules on Gene Expression -- A Method to Isolate, Purify, and Characterize Human Periodontal Ligament Stem Cells -- Preclinical Methods for the Evaluation of Periodontal Regeneration In Vivo -- Proteomic Analysis of Dental Tissue Microsamples -- Immunological Techniques: ELISA, Flow Cytometry, and Immunohistochemistry -- Analysis of Immune Responses to Purified Recombinant Antigens of Periodontal Pathogens -- Single-Strand Conformation Polymorphism Analysis for the Diagnosis of T-Cell Clonality in Periodontal Disease -- Real-Time PCR Focused-Gene Array Profiling of Gingival and Periodontal Ligament Fibroblasts -- The Use of Gene Arrays in Deciphering the Pathobiology of Periodontal Diseases -- Bioinformatics Techniques in Microarray Research: Applied Microarray Data Analysis Using R and SAS Software.
520 _aWith so many major advances over the past two decades coming through the application of molecular biology and nanotechnology, it is essential that dental research, education, and practice keep pace with the rapid progress of science. In Oral Biology: Molecular Techniques and Applications, expert researchers in the field have provided a selection of in depth methods and techniques optimized for particular applications, which can be readily adapted to particular organisms or areas of interest. Divided into three convenient sections, the book covers the study of saliva, as it is a rich source of biomolecules for study at the molecular level, which may lead to the identification of susceptibility to particular diseases, the study of the microbial inhabitants that share the oral cavity with us, as well as a range of protocols that facilitate assessment of the molecular behavior of oral cells and tissues in health and during disease progression. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Oral Biology: Molecular Techniques and Applications is an ideal resource not only to the new researcher but also to the seasoned laboratory veteran including cell biologists, microbiologists, and any researchers intent on delving into the vital world of oral biology.
988 _aSpringer_Protocols_2010
650 7 _2embne
_9168146
_aMicrobiología oral
776 0 8 _iPrinted edition:
_z9781607618195
776 0 8 _iPrinted edition:
_z9781607618218
776 0 8 _iPrinted edition:
_z9781493961429
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-820-1
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b12/2023
_dz
_ean
_zSI