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008			100301s2002 xxu| fo |||| 0|eng d
020			_a9781592592838
024	7		_a10.1385/159259283X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP606 .D46 _b2002 EB
245	0	0	_aRT-PCR Protocols _cedited by Nicola King, Joe O'Connell.
250			_a1st edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (XIV, 382 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v193
505	0		_aRT-PCR in Biomedicine -- The Basics of RT-PCR -- Highly Sensitive Detection and Analysis of mRNA -- Using the Quantitative Competitive RT-PCR Technique to Analyze Minute Amounts of Different mRNAs in Small Tissue Samples -- Detection of mRNA Expression and Alternative Splicing in a Single Cell -- Nested RT-PCR -- Quantitative RT-PCR -- Quantitative RT-PCR -- Rapid Development of a Quantitative-Competitive (qc) RT-PCR Assay Using a Composite Primer Approach -- Quantitation of Gene Expression by RT-PCR and HPLC Analysis of PCR Products -- Time-Resolved Fluorometric Detection of Cytokine mRNAs Amplified by RT-PCR -- Mimic-Based RT-PCR Quantitation of Substance P mRNA in Human Mononuclear Phagocytes and Lymphocytes -- Detection and Analysis of RNA Viruses -- Detection and Quantification of the Hepatitis C Viral Genome -- Semi-Quantitative Detection of Hepatitis C Virus RNA by "Real-Time" RT-PCR -- RT-PCR for the Assessment of Genetically Heterogenous Populations of the Hepatitis C Virus -- In Situ Localization of mRNA Expression -- in situ Immuno-PCR -- RT-PCR from Laser-Capture Microdissected Samples -- Mycobacterium paratuberculosis Detected by Nested PCR in Intestinal Granulomas Isolated by LCM in Cases of Crohn's Disease -- RT-PCR-Based Approaches to Generate Probes for mRNA Detection by In Situ Hybridization -- Differential mRNA Expression -- Amplified RNA for Gene Array Hybridizations -- Semi-Quantitative Determination of Differential Gene Expression in Primary Tumors and Matched Metastases by RT-PCR -- Genetic Analysis -- Detection of Single Nucleotide Polymorphisms Using a Non-Isotopic RNase Cleavage Assay -- RT-PCR in Immunology -- Detection of Clonally Expanded T-Cells by RT-PCR-SSCP and Nucleotide Sequencing of T-Cell Receptor ?-CDR3 Regions -- Generation of scFv from a Phage Display Mini-Library Derived from Tumor-Infiltrating B-Cells -- Generation of Murine scFv Intrabodies from B-Cell Hybridomas -- Quantitation of mRNA Levels by RT-PCR in Cells Purified by FACS -- RT-PCR in Anti-Sense Technology -- Detection of Anti-Sense RNA Transcripts by Anti-Sense RT-PCR -- RT-PCR in cDNA Cloning -- RT-PCR in cDNA Library Construction -- An RT-PCR-Based Protocol for the Rapid Generation of Large, Representative cDNA Libraries for Expression Screening.
520			_aRT-PCR, the most widely used technique in biomedical research for analyzing gene expression, has proven a significant boon to the molecular investigation of disease pathogenesis. In RT-PCR Protocols, Joe O'Connell has assembled a panel of highly regarded molecular biologists and clinical researchers to describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering. Each protocol emphasizes the technical steps critical for experimental success and includes tips on avoiding potential pitfalls, notes explaining the method's scientific basis and its possible sources of error, and comments on how it might best be accommodated to various experimental circumstances. Diverse and wide-ranging, RT-PCR Protocols offers investigators powerful techniques to be used in the molecular investigation of disease pathogenesis and shows how RT-PCR can complement other technological advances in the design of today's new therapeutic strategies.
988			_aSpringer_Protocols_2002
650		7	_2embne _9138640 _aEnzimas
776	0	8	_iPrinted edition: _z9781617372322
776	0	8	_iPrinted edition: _z9780896038752
776	0	8	_iPrinted edition: _z9781489938800
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159259283X _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI