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008			100301s2003 xxu| fo |||| 0|eng d
020			_a9781592593569
024	7		_a10.1385/1592593569 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC269.7 _b2003 EB
245	0	0	_aCancer Cell Signaling _b: Methods and Protocols _cedited by David M. Terrian.
250			_a1st edition 2003
264		1	_aTotowa, NJ _bHumana Press _c2003
300			_a1 recurso en línea (XV, 333 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v218
505	0		_aManipulation and Detection of Survival Signals -- Functional Analysis of the Antimitogenic Activity of Tumor Suppressors -- Rescue and Isolation of Rb-deficient Prostate Epithelium by Tissue Recombination -- Signal Transduction Study Using Gene-Targeted Embryonic Stem Cells -- The Use of the Yeast Two-Hybrid System to Measure Protein-Protein Interactions that Occur Following Oxidative Stress -- Differential Screening of cDNA Libraries for Analysis of Gene Expression During Tumor Progression -- Mitogen-Activated Protein Kinase Signaling in Drug-Resistant Neuroblastoma Cells -- TUNEL and Immunofluorescence Double-Labeling Assay for Apoptotic Cells with Specific Antigen(s) -- Manipulation and Edtection of Oncogenic Signals -- Kinetworks™ Protein Kinase Multiblot Analysis -- Protein Tyrosine Kinase and Phosphatase Expression Profiling in Human Cancers -- Association of Nonreceptor Tyrosine Kinase c-Yes with Tight Junction Protein Occludin by Coimmunoprecipitation Assay -- Isolation of Novel Substrates Using a Tyrosine Kinase Overlay/In Situ Assay -- Manipulating Expression of Endogenous Oncogenic Proteins Using an Antisense Oligonucleotide Approach in Prostate Cancer Cells -- Measurements of Phospholipases A2, C, and D (PLA2, PLC, and PLD) -- Detection of the Content and Activity of the Transcription Factor AP-1 in a Multistage Skin Carcinogenesis Model -- Fibroblastic, Hematopoietic, and Hormone Responsive Epithelial Cell Lines and Culture Conditions for Elucidation of Signal Transduction and Drug Resistance Pathways by Gene Transfer -- Elucidation of Signal Transduction Pathways by Transfection of Cells with Modified Oncogenes -- Elucidation of Signal Transduction Pathways by Retroviral Infection of Cells with Modified Oncogenes -- Protein Interactions -- Methods for the Study of Protein-Protein Interactions in Cancer Cell Biology -- Production of Ligand-Specific Mutants Using a Yeast Two-Hybrid Mating Assay -- Coimmunoprecipitation Assay for the Detection of Kinase-Substrate Interactions -- Genomic Rearrangements -- Mutational Analysis of the Androgen Receptor Using Laser Capture Microdissection and Direct Sequencing -- Clonality Analysis by T-Cell Receptor ? PCR and High-Resolution Electrophoresis in the Diagnosis of Cutaneous T-Cell Lymphoma (CTCL).
520			_aThe rapidly evolving nature of cell signaling research in cancer has necessitated a continuous updating of the methods used in its study, most recently to take into account the analysis of multiple members within a gene family. Cancer Cell Signaling: Methods and Protocols brings together all the major methods of cell research that are scientifically grounded within the cancer biology field. Presented in step-by-step detail to ensure successful results, the methods include proven techniques for the investigation of apoptosis and cell death, complementary protocols for manipulating and/or monitoring oncogenic signals in cancer cells, and techniques for studying protein-protein interactions. Finally, there is a detailed protocol for capturing pure samples of malignant cells from frozen tissue specimens and two alternative techniques for analyzing their genomic DNA. Each method is described by a hands-on master of the technique and includes invaluable notes on troubleshooting and pitfalls to avoid. Cutting-edge and highly practical, Cancer Cell Signaling: Methods and Protocols provides today's cancer researchers with new, but well-proven, strategies and procedures for investigating how the signaling cascades essential to normal cell life are corrupted, as well as for developing new molecular tools for cancer therapy.
988			_aSpringer_Protocols_2003
650		7	_2embne _9146156 _aCélulas cancerosas
776	0	8	_iPrinted edition: _z9781617373411
776	0	8	_iPrinted edition: _z9781588290755
776	0	8	_iPrinted edition: _z9781489938084
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592593569 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI