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008			100301s2003 xxu| fo |||| 0|eng d
020			_a9781592593743
024	7		_a10.1385/1592593747 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRB131 _b2003 EB
245	0	0	_aInflammation Protocols _cedited by Paul G. Winyard, Derek A. Willoughby.
250			_a1st edition 2003
264		1	_aTotowa, NJ _bHumana Press _c2003
300			_a1 recurso en línea (XII, 380 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v225
505	0		_aIn Vitro Systems for Studying Aspects of the Inflammatory Response and Testing Antiinflammatory Drugs -- Key Stages in the Acute Inflammatory Response and Their Relevance as Therapeutic Targets -- I?B Kinase and NF-?B Signaling in Response to Pro-Inflammatory Cytokines -- Screening for Inhibitors of Transcription Factors Using Luciferase Reporter Gene Expression in Transfected Cells -- Adhesion Molecule Expression on Cytokine-Stimulated Human Endothelial Cells -- Phagocytosis by Inflammatory Phagocytes -- Cytosolic Ca2+ Measurement and Imaging in Inflammatory Cells -- Detection and Visualization of Oxidase Activity in Phagocytes -- Measurement of Complement Activation -- Measurement of Matrix Metalloproteinase Activities in the Medium of Cultured Synoviocytes Using Zymography -- Measurement of Aggrecanase-Generated Interglobular Domain Catabolites in the Medium and Extracts of Cartilage Explants Using Western Blot Analysis -- In Vitro Model of Human Articular Cartilage Degradation -- In Vivo Models of Inflammation -- In Vivo Models of Inflammation -- Carrageenan-Induced Paw Edema in the Rat and Mouse -- Pleural Models of Inflammation -- Models of Acute Inflammation in the Ear -- Migration of Specific Leukocyte Subsets in Response to Cytokine or Chemokine Application In Vivo -- Inflammatory Joint Disease -- The Assessment of Inflammation, Cartilage Matrix, and Bone Loss in Experimental Monoarticular Arthritis of the Rat -- Collagen-Induced Arthritis -- Air-Pouch Models of Inflammation and Modifications for the Study of Granuloma-Mediated Cartilage Degradation -- Quantitative Analysis of Angiogenesis Using the Murine Chronic Granulomatous Air Pouch -- Models of Coronary Artery Occlusion and Reperfusion for the Discovery of Novel Antiischemic and Antiinflammatory Drugs for the Heart -- Assessment of Anticolitic Drugs in the Trinitrobenzene Sulfonic Acid (TNBS) Rat Model of Inflammatory Bowel Disease -- An In Vivo Model of Ischemia/Reperfusion and Inflammation of the Kidneys of the Rat -- In Vivo Models of Inflammation -- Wound Healing -- Pharmacodynamic Endpoints in Experimental Models and In Clinical Studies in Humans -- An Iconoclastic Approach to Pharmacodynamics in Model Systems: Their Relevance to Humans -- A Reply to "An Iconoclastic Approach to Pharmacodynamics in Model Systems: Their Relevance to Humans" -- Quantifying Inflammation In Vivo Using Radiolabeled Antibodies and Leukocytes -- Immunoperoxidase Histochemistry for the Detection of Cellular Adhesion Molecule, Cytokine, and Chemokine Expression in the Arthritic Synovium -- Roles of Nitric Oxide and Superoxide in Inflammation -- Analysis of Nitrite and Nitrate in the Study of Inflammation -- In Vivo Assays for COX-2 -- Measurement of 8-epi-PGF2? as a Marker of Lipid Peroxidation In Vivo by Immunoaffinity Extraction and Gas Chromatography-Mass Spectrometry -- Laboratory Assessment of the Acute Phase Response -- Assays of Matrix Metalloproteinases (MMPs) and MMP Inhibitors.
520			_aBecause inflammation is involved in many chronic human diseases, its proper understanding may provide important clues to new therapeutic targets. In Inflammation Protocols, highly skilled experimenters present key techniques for the multidisciplinary study of inflammation in such conditions as inflammatory bowel disease, rheumatoid arthritis, renal disease, and cardiovascular disease. Described in step-by-step detail to ensure experimental success, the techniques are focused on the research and development of potential new antiinflammatory drugs in active target areas. The protocols are suitable for many current areas of drug discovery research, including transcription factors, cytokines, adhesion molecules, cyclooxygenase-2 (COX-2) inhibitors, free radicals, nitric oxide synthases, complement activation, angiogenesis, wound healing, immune rejection, and metalloproteinases. The methods provide in vitro systems for studying inflammation, in vivo models, and relevant pharmacodynamic measurements for the assessment of antiinflammatory compounds. Each protocol contains notes about potential pitfalls and tips on how to avoid failure. Overview chapters at the start of each section review the significance and potential limitations of the protocols presented. Cutting-edge and highly practical, Inflammation Protocols offers experimental and clinical researchers a premier collection of readily reproducible techniques not only for evaluating inflammatory disease processes, but also for the discovery of new drugs for treating chronic human inflammatory diseases today.
988			_aSpringer_Protocols_2003
650		7	_2embne _9140839 _aAntiinflamatorios
776	0	8	_iPrinted edition: _z9781617372827
776	0	8	_iPrinted edition: _z9780896039704
776	0	8	_iPrinted edition: _z9781489938268
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592593747 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI