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008			100301s2009 xxu| fo |||| 0|eng d
020			_a9781603270199
024	7		_a10.1007/978-1-60327-019-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH442.6 _b2009 EB
245	0	0	_aTransgenesis Techniques _b: Principles and Protocols _cedited by Elizabeth J. Cartwright.
250			_a3rd edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XII, 335 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v561
505	0		_aTransgenesis in Various Model Systems -- Transgenesis in Drosophila melanogaster -- Transgenesis in Caenorhabditis elegans -- Transgenesis in Zebrafish with the Tol2 Transposon System -- Generation of Transgenic Frogs -- Pronuclear DNA Injection for the Production of Transgenic Rats -- Transgenesis in the Mouse -- Cell-Type-Specific Transgenesis in the Mouse -- Transgene Design and Delivery into the Mouse Genome: Keys to Success -- Overexpression Transgenesis in Mouse: Pronuclear Injection -- Gene-Targeting Vectors -- Gene Trap: Knockout on the Fast Lane -- Culture of Murine Embryonic Stem Cells -- Targeting Embryonic Stem Cells -- Generation of Chimeras by Microinjection -- Generation of Chimeras by Morula Aggregation -- Surgical Techniques for the Generation of Mutant Mice -- Site-Specific Recombinases for Manipulation of the Mouse Genome -- Cre Transgenic Mouse Lines -- Large-Scale Mouse Mutagenesis -- Dedicated Mouse Production and Husbandry -- Biological Methods for Archiving and Maintaining Mutant Laboratory Mice. Part I: Conserving Mutant Strains -- Biological Methods for Archiving and Maintaining Mutant Laboratory Mice. Part II: Recovery and Distribution of Conserved Mutant Strains.
520			_aOne of the major challenges currently facing the scientific community is to understand the function of the multitude of protein-coding genes that were revealed when the human genome was fully sequenced. In Transgenesis Techniques: Principles and Protocols, Third Edition, experts in the field fully update and expand upon the previous edition in order to detail the transgenic techniques currently used to modify the genome. The volume explores classic procedures to genetically modify mice and other model organisms, as well as cutting-edge practices involving microinjection, site-specific recombination systems, cryopreservation, and many other topics. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenesis Techniques: Principles and Protocols, Third Edition is a comprehensive practical guide to the generation of transgenic animals and an invaluable source of information in any lab currently involved in transgenic techniques.
988			_aSpringer_Protocols_2009
650		7	_2embne _9141520 _aIngeniería genética
776	0	8	_iPrinted edition: _z9781607611134
776	0	8	_iPrinted edition: _z9781603270182
776	0	8	_iPrinted edition: _z9781627038416
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-019-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI