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007			cr nn 008mamaa
008			100301s1998 xxu| fo |||| 0|eng d
020			_a9781592595730
024	7		_a10.1385/0896034380 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP801. R47 _b1998 EB
245	0	0	_aRetinoid Protocols _cedited by Christopher Redfern.
250			_a1st edition 1998
264		1	_aTotowa, NJ _bHumana Press _c1998
300			_a1 recurso en línea (XVI, 434 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v89
505	0		_aHandling and Analysis of Retinoids -- Properties of Retinoids -- Quantitative Analyses of Naturally Occurring Retinoids -- Detection and Measurement of Retinoic Acid Production by Isolated Tissues Using Retinoic Acid-Sensitive Reporter Cell Lines -- Retinoid-Binding Proteins -- Immunohistochemistry for CRBPs and CRABPs -- Whole-Mount In Situ Hybridization of Mouse Embryos Exposed to Teratogenic Levels of Retinoic Acid -- Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) for Cellular Retinoid-Binding Proteins -- Methods for Producing Recombinant Human Cellular Retinaldehyde-Binding Protein -- Expression and Purification of CRABPs from E. coli -- Purification and Fluorescent Titration of Cellular Retinol-Binding Protein -- Fluorometric Titration of the CRABPs -- Expression and Mutagenesis of Retinol-Binding Protein -- Interactions of Retinol-Binding Protein with Transthyretin and Its Receptor -- Detection of Conformational Changes in Cellular Retinoid-Binding Proteins by Limited Proteolysis -- Measurement of Rates of Dissociation of Retinoids from the Interphotoreceptor Retinoid-Binding Protein -- Use of Antisense Oligonucleotides to Study the Role of CRABPs in Retinoic Acid-Induced Gene Expression -- Nuclear Retinoid Receptors -- Preparation of Polyclonal Antibodies to Retinoid Receptors -- Detection of RARs and RXRs in Cells and Tissues Using Specific Ligand-Binding Assays and Ligand-Binding lmmunoprecipitation Techniques -- Nonisotopic In Situ Hybridization for the Detection of Nuclear Retinoid Receptor Transcripts in Tissue Sections -- In Situ Hybridization with 35S-Labeled Probes for Retinoid Receptors -- Isolation of Retinoid Receptors from Manimalian Cells -- Analysis of Retinoid Receptor Phosphorylation -- Photoaffinity Labeling of RARs and Mapping of Labeled Sites by an Endoproteinase Combination Technique -- PCR Cloning of N-Terminal RAR Isoforms and APL-Associated PLZF-RAR? Fusion Proteins -- RT-PCR in Diagnosis and Disease Monitoring of Acute Promyelocytic Leukemia (APL) -- A Two-Hybrid Protein Interaction System to Identify Factors That Interact with Retinoid and Vitamin D Receptors -- Gel-Shift Analysis and Identification of RXREs and RAREs by PCR-Based Selection -- Identification and Cloning of RA-Regulated Genes by mRNA-Differential Display -- Gene Targeting of Retinoid Receptors.
520			_aChristopher Redfern brings together in Retinoid Protocols a comprehensive collection of key biochemical and molecular methods for retinoid research. These easily reproducible techniques range from methods of handling and analyzing retinoids to advanced protocols for gene targeting, from binding protein function in transgenic animals to the study of nuclear retinoid receptors. In addition, the book makes accessible to all retinoid researchers such advanced techniques as cloning via RT-PCR, fluorimetry methods, recombinant protein purification and characterization, the use of antisense oligonucleotides to study the role of CRABPs, immunological and in situ hybridization methods, photoaffinity labeling, gel-shift analysis, and differential display. Many of the chapters in Retinoid Protocols represent landmarks in retinoid methodology because they give step-by-step instructions for techniques central to understanding cellular control by retinoids. These indispensable techniques will be fundamental for all those working with retinoids, and so will play a key role in the development of new drugs and therapies for treating human disease.
988			_aSpringer_Protocols_1998
650		7	_2embne _9157930 _aRetinoides
776	0	8	_iPrinted edition: _z9781489942449
776	0	8	_iPrinted edition: _z9780896034389
776	0	8	_iPrinted edition: _z9781489942432
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896034380 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI