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008			140509s2014 xxu| fo |||| 0|eng d
020			_a9781493904464
024	7		_a10.1007/978-1-4939-0446-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aSB123 _b2014 EB
245	0	0	_aCrop Breeding _b: Methods and Protocols _cedited by Delphine Fleury, Ryan Whitford.
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (X, 255 páginas) _b67 ilustraciones, 58 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1145
505	0		_aDefining a Genetic Ideotype for Crop Improvement -- From Genes to Markers: Exploiting Gene Sequence Information to Develop Tools for Plant Breeding -- Temperature Switch PCR (TSP) - A Gel-Based Molecular Marker Technique for Investigating Single Nucleotide Polymorphisms -- Multiplex-Ready Technology for Mid-Throughput Genotyping of Molecular Markers -- Genotyping by High-Resolution Melting Analysis -- Bi-Allelic SNP Genotyping Using the TaqMan® Assay -- SNP Genotyping: The KASPTM Assay -- Rindsel: An R Package for Phenotypic and Molecular Selection Indices Used in Plant Breeding -- OptiMAS: A Decision Support Tool to Conduct Marker-Assisted Selection Programs -- Genomic Selection in Plant Breeding -- Simulated Breeding with QU-GENE Graphical User Interface -- The Control of Recombination in Wheat by Ph1 and Its Use in Breeding -- TILLING for Plant Breeding -- In vitro Culture for Doubled Haploids: Tools for Molecular Breeding -- Biolistic Transformation of Wheat with Centrophenoxine as a Synthetic Auxin -- Agrobacterium-Mediated Transformation of Barley (Hordeum vulgare L.) -- qPCR for Quantification of Transgene Expression and Determination of Transgene Copy Number -- High-Throughput Analysis Pipeline for Achieving Simple Low-Copy Wheat and Barley Transgenics.
520			_aThe recent advent of molecular technologies has changed the way plant breeders identify and select their germplasm as genetic variation can now be assessed at the DNA level. Crop Breeding: Methods and Protocols presents detailed guidelines and tutorials that suit different needs and capacity from small laboratories analyzing molecular markers on a one-by-one basis to the increasingly popular high-throughput protocols for high capacity laboratories. Topics covered include breeding strategy for the selection of an ideal variety or genetic ideotype, protocols for breeders using molecular markers in selection programs and for laboratories providing molecular services to breeding programs, statistical programs and software to aid implementation of molecular data into breeding programs, and methodologies that facilitate the generation of genetic diversity and its characterization. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Crop Breeding: Methods and Protocols will help in expanding the use of molecular technologies for the creation of tomorrow's crop varieties.
988			_aSpringer_Protocols_2014
650		7	_2embne _9138492 _aBotánica
776	0	8	_iPrinted edition: _z9781493904471
776	0	8	_iPrinted edition: _z9781493904457
776	0	8	_iPrinted edition: _z9781493943241
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-0446-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI