000			04847nam a22003975i 4500
999			_c393314 _d393314
001			393314
003			ES-MaUEC
005			20240217101915.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			141127s2015 xxu| fo |||| 0|eng d
020			_a9781493921522
024	7		_a10.1007/978-1-4939-2152-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP356.2 _b2015 EB
245	0	0	_aNeuronal Cell Death _b: Methods and Protocols _cedited by Laura Lossi, Adalberto Merighi.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XIV, 396 páginas) _b82 ilustraciones, 48 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1254
505	0		_aNeuronal Cell Death: An Overview of its Different Forms in Central and Peripheral Neurons -- Cell Volume Regulation Monitored with Combined Epifluorescence and Digital Holographic Microscopy -- Flow Cytometric Analysis of DNA Synthesis and Apoptosis in Central Nervous System Using Fresh Cell Nuclei -- Nuclear Signs of Pre-Neurodegeneration -- Multi-Parametric O2 Imaging in Three-Dimensional Neural Cell Models with the Phosphorescent Probes -- Calcium Imaging in Neuron Cell Death -- Monitoring Mitochondrial Membranes Permeability in Live Neurons and Mitochondrial Swelling through Electron Microscopy Analysis -- Real-Time Visualization of Caspase-3 Activation by Fluorescence Resonance Energy Transfer (FRET) -- Design and Cloning of Short Hairpin RNAs (shRNAs) into a Lentiviral Silencing Vector to -- Genomic Analysis Using Affymetrix Standard Microarray GeneChips (169 Format) in Degenerate Murine Retina -- Genomic Analysis of Transcriptional Changes Underlying Neuronal Apoptosis -- High-Throughput Cell Death Assays -- Image Analysis Algorithms for Immunohistochemical Assessment of Cell Death -- In Vitro Oxygen-Glucose Deprivation to Study Ischemic Cell Death -- Laser Microbeam Targeting of Single Nerve Axons in Cell Culture -- Real-Time Imaging of Retinal Cell Apoptosis by Confocal Scanning Laser Ophthalmoscopy -- Targeted Toxicants to Dopaminergic Neuronal Cell Death -- Stem Cells, Neural Progenitors, and Engineered Stem Cells -- Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant Vectors for Gene Transfer and Gene Therapy -- Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors for Gene Transfer and Gene Therapy -- Bone Marrow Transplantation for Research and Regenerative Therapies in the Central Nervous System -- Detection of Activated Caspase-8 in Injured Spinal Axons by Using Fluorochrome-Labeled Inhibitors of Caspases (FLICA) -- Generation of Zebrafish Models by CRISPR/Cas9 Genome Editing -- In Vivo Assessment of Neuronal Cell Death in Drosophila -- Drosophila Model for Studying Phagocytosis Following Neuronal Cell Death.
520			_aThis volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research. Conveniently divided into four parts, sections cover a series of techniques for the molecular, structural, functional and genomic characterization of dying neurons, a number of protocols that are of primary interest in neuropathology and in experimental neuropathology, a series of gene engineering techniques to obtain and manipulate neuronal stem cells and progenitors, to prepare HSV-1 vectors for the gene therapy, and to CNS transplantation of bone marrow stem cells, and finally, some very interesting protocols for the study of cell death in non-mammalian models. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Neuronal Cell Death: Methods and Protocols seeks to serve a large audience of scientists that are currently active in the field or are willing to enter such an exciting and still expanding area of neurobiology.
988			_aSpringer_Protocols_2015
650		7	_2embne _9144348 _aNeuronas
776	0	8	_iPrinted edition: _z9781493921539
776	0	8	_iPrinted edition: _z9781493921515
776	0	8	_iPrinted edition: _z9781493944644
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2152-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI