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008			151001s2015 xxu| fo |||| 0|eng d
020			_a9781493928712
024	7		_a10.1007/978-1-4939-2871-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552 .M44 _b2015 EB
245	0	4	_aThe BAM Complex _b: Methods and Protocols _cedited by Susan Buchanan, Nicholas Noinaj.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XI, 283 páginas) _b43 ilustraciones, 16 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1329
505	0		_aThe β-barrel Assembly Machinery Comple -- Yeast Mitochondria as a Model System to Study the Biogenesis of Bacterial β-barrel Proteins -- Experimental Methods for Studying the BAM Complex in Neisseria meningitides -- Heat Modifiability of Outer Membrane Proteins from Gram-Negative Bacteria -- The Role of a Destabilized Membrane for OMP Insertion -- Treponema pallidum in Gel Microdroplets: A Method for Topological Analysis of BamA (TP0326) and Localization of Rare Outer Membrane Proteins -- Analyzing the Role of Periplasmic Folding Factors in the Biogenesis of OMPs and Members of the Type V Secretion System -- An In Vitro Assay for Substrate Translocation by FhaC in Liposomes -- Measuring Cell-cell Binding Using Flow-Cytometry -- Methods to Characterize Folding and Function of BamA Crosslink Mutants -- Small Angle X-ray Scattering (SAXS) Characterization of the POTRA Domains of BamA -- Assessing the Outer Membrane Insertion and Folding of Multimeric Transmembrane β-barrel Proteins -- The Expression, Purification, and Structure Determination of BamA from E. coli -- Expression and Purification of the Individual Bam Components BamB-E -- Structure Determination of the BAM Complex Accessory Lipoproteins BamB-E -- An In Vitro Assay for Outer Membrane Protein Assembly by the BAM complex -- Identification of BamC on the Surface of E. coli -- Construction and Characterization of an E. coli bamD Depletion Strain -- Expression, Purification and Screening of BamE, a Component of the BAM Complex, for Structural Characterization -- Purification and Bicelle Crystallization for Structure Determination of the E. coli Outer Membrane Protein TamA -- Strategies for the Analysis of Bam Recognition Motifs in Outer Membrane Proteins -- Summary and Future Directions.
520			_aThis volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex.
988			_aSpringer_Protocols_2015
650		7	_2embne _9671893 _aProteínas de membranas
776	0	8	_iPrinted edition: _z9781493928705
776	0	8	_iPrinted edition: _z9781493928729
776	0	8	_iPrinted edition: _z9781493949205
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2871-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI