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008			100301s1999 xxu| s |||| 0|eng d
020			_a9781592592708
024	7		_a10.1385/1592592708 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQL959 _b1999 EB
245	0	0	_aMolecular Embryology _cedited by Paul T. Sharpe, Ivor Mason
250			_a1st edition 1999
264		1	_aTotowa, NJ _bHumana Press _c1999
300			_a1 recurso en línea (XVI, 756 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v97
505	0		_aThe Mouse Embryo -- The Mouse as a Developmental Model -- Culture of Postimplantation Mouse Embryos -- Organ Culture in the Analysis of Tissue Interactions -- Treatment of Mice with Retinoids In Vivo and In Vitro -- Cell Grafting and Labeling in Postimplantation Mouse Embryos -- Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-Celled Eggs -- Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells -- Gene Trapping in Mouse Embryonic Stem Cells -- Production and Growth of Conditionally Immortal Cell Lines from the H-2Kb tsA58 Transgenic Mouse -- Reporter Genes for the Study of Transcriptional Regulation in Transgenic Mouse Embryos -- Application of lacZ Transgenic Mice to Cell Lineage Studies -- Mouse Primordial Germ Cells -- Chicken Embryo -- The Avian Embryo -- Chick Embryos -- New Culture -- Grafting Hensen's Node -- Grafting of Somites -- Notochord Grafts -- Transplantation of Avian Neural Tissue -- Grafting of Apical Ridge and Polarizing Region -- Tissue Recombinations in Collagen Gels -- Quail-Chick Chimeras -- Using Fluorescent Dyes for Fate Mapping, Lineage Analysis, and Axon Tracing in the Chick Embryo -- Amphibian Embryo -- An Overview of Xenopus Development -- Mesoderm Induction Assays -- Experimental Embryological Methods for Analysis of Neural Induction in the Amphibian -- A Method for Generating Transgenic Frog Embryos -- Axolotl/newt -- Zebrafish -- The Zebrafish -- Small-Scale Marker-Based Screening for Mutations in Zebrafish Development -- Transgenic Zebrafish -- Microinjection of DNA, RNA, and Protein into the Fertilized Zebrafish Egg for Analysis of Gene Function -- Retinoids in Nonmammalian Embryos -- Monvertevrate Chordates -- Protochordates -- Retroviruses -- Gene Transfer to the Rodent Embryo by Retroviral Vectors -- Gene Transfer in Avian Embryos Using Replication-Competent Retroviruses -- Molecular Techniques -- Subtractive Hybridization and Construction of cDNA Libraries -- Differential Display of Eukaryotic mRNA -- RT-PCR on Embryos Using Degenerate Oligonucleotide Primers -- Single-Cell RT-PCR cDNA Subtraction -- In Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections -- In Situ Hybridization to RNA in Whole Embryos -- Wholemount In Situ Hybridization to Xenopus Embryos -- Wholemount In Situ Hybridization to Amphioxus Embryos -- In Situ Hybridization to Sections (Nonradioactive) -- Immunohistochemistry Using Polyester Wax -- Immunohistochemistry on Whole Embryos -- Whole Embryo Assays for Programmed Cell Death -- Gene Interference Using Antisense Oligodeoxynucleotides on Whole Chick Embryos -- Protein Techniques -- Microscopy and Photography -- Microscopy and Photomicrography Techniques.
520			_aIn Molecular Embryology, expert investigators provide a comprehensive guide to the cutting-edge methods used today across the dramatically growing field of vertebrate molecular embryology. These powerful techniques take advantage of the most commonly used vertebrate experimental models: murine embryos for their genetics, chick embryos for in vivo manipulation, zebrafish for mutagenesis, amphibian embryos, and nonvertebrate chordates. The major techniques of experimental molecular biology and the particular advantages of each different species are emphasized. Detailed, easy-to-follow protocols, together with relevant background information and helpful tips, optimize the methods for success. Molecular Embryology brings together in one volume all the major techniques and common experimental species needed to study the mechanisms of biological development in vertebrates. Bound to become a standard reference in this field, the book makes it possible for experienced and novice researchers alike to move between embryos of diverse vertebrate classes as their project progresses, ensuring their ability to utilize the experimental advantages of different systems to address specific developmental questions.
988			_aSpringer_Protocols_1999
650		7	_2embne _9140065 _aEmbriología
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592708 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ejc _zSI